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Dear Abcam technical support,
Thank you for offering your help in testing the different lots of this antibody with the mouse lung. Certainly, I would like to see if your lab can find out any lot of this antibody that can produce the apical staining that I showed you in my previous email. We have found another nucleolin antibody that give us some of the staining we wanted but still didn't work as well as we expected. Also, since this staining pattern was published on Nature and your antibody was referenced, we would still want to produce consistent results with the same ab22758 antibody. Please inform me when you have the results and I'm looking forward to see if we can get the apical staining back.
Really appreciate for your help and please let me know if I might be helpful to provide you additional information to assist your test on the antibody.
Asked on Jun 05 2012
Thank you for your reply.
I have let the lab know that they should re-test the antibody with mouse lung samples, and I will let you know as soon as I have the data. I will also send on the previous IHC images once I have access to them.
Please let me know if there is anything else that we can do. I am sorry again for the batch variability, but hopefully this will be resolved shortly.
Have a great day.
Answered on Jun 05 2012