Recombinant Anti-Nucleolin antibody [EPR7951] (ab134164)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7951] to Nucleolin
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Nucleolin antibody [EPR7951]
See all Nucleolin primary antibodies -
Description
Rabbit monoclonal [EPR7951] to Nucleolin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, HuT-78, HeLa, and Jurkat cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Dissociation constant (KD)
KD = 2.90 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7951 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 76 kDa.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 76 kDa. |
Target
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Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Post-translational
modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 4691 Human
- Entrez Gene: 17975 Mouse
- Entrez Gene: 25135 Rat
- Omim: 164035 Human
- SwissProt: P19338 Human
- SwissProt: P09405 Mouse
- SwissProt: P13383 Rat
- Unigene: 79110 Human
see all -
Alternative names
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
Images
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All lanes : Anti-Nucleolin antibody [EPR7951] (ab134164) at 1/10000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : Nucleolin knockout HeLa whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 76 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab134164 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab134164 was shown to specifically react with Nucleolin in wild-type HeLa cells as signal was lost in Nucleolin knockout cells. Wild-type and Nucleolin knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab134164 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Nucleolin antibody [EPR7951] (ab134164) at 1/1000 dilution
Lane 1 : K562 cell lysates
Lane 2 : HuT-78 cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 76 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab134164 has been referenced in 3 publications.
- Huang YH et al. MicroRNA-29a is a key regulon that regulates BRD4 and mitigates liver fibrosis in mice by inhibiting hepatic stellate cell activation. Int J Med Sci 16:212-220 (2019). PubMed: 30745801
- Di Tomasso G et al. ARiBo pull-down for riboproteomic studies based on label-free quantitative mass spectrometry. RNA 22:1760-1770 (2016). WB ; Human . PubMed: 27659051
- Chang YC et al. An XIST-related small RNA regulates KRAS G-quadruplex formation beyond X-inactivation. Oncotarget 7:86713-86729 (2016). PubMed: 27880931