Overview

  • Product name

    Anti-Nucleolin antibody [EPR7951]
    See all Nucleolin primary antibodies
  • Description

    Rabbit monoclonal [EPR7951] to Nucleolin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminal in Human Nucleolin (P19338).

  • Positive control

    • K562, HuT-78, HeLa, and Jurkat cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 76 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
    • Sequence similarities

      Contains 4 RRM (RNA recognition motif) domains.
    • Post-translational
      modifications

      Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
    • Cellular localization

      Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • Database links

    • Alternative names

      • C23 antibody
      • FLJ45706 antibody
      • MS1116 antibody
      • NCL antibody
      • Nucl antibody
      • NUCL_HUMAN antibody
      • Nucleolin antibody
      • Protein C23 antibody
      see all

    Images

    • All lanes : Anti-Nucleolin antibody [EPR7951] (ab134164) at 1/10000 dilution

      Lane 1 : Wild-type HeLa whole cell lysate
      Lane 2 : Nucleolin knockout HeLa whole cell lysate
      Lane 3 : MCF7 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 76 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?



      Lanes 1 - 3: Merged signal (red and green). Green - ab134164 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab134164 was shown to specifically react with Nucleolin in wild-type HeLa cells as signal was lost in Nucleolin knockout cells. Wild-type and Nucleolin knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab134164 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Nucleolin antibody [EPR7951] (ab134164) at 1/1000 dilution

      Lane 1 : K562 cell lysates
      Lane 2 : HuT-78 cell lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 76 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Huang YH  et al. MicroRNA-29a is a key regulon that regulates BRD4 and mitigates liver fibrosis in mice by inhibiting hepatic stellate cell activation. Int J Med Sci 16:212-220 (2019). Read more (PubMed: 30745801) »
    • Di Tomasso G  et al. ARiBo pull-down for riboproteomic studies based on label-free quantitative mass spectrometry. RNA 22:1760-1770 (2016). WB ; Human . Read more (PubMed: 27659051) »
    See all 3 Publications for this product

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