Anti-Nucleolin antibody [SPM614] (ab233945)

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Overview

  • Product name

    Anti-Nucleolin antibody [SPM614]
    See all Nucleolin primary antibodies

  • Description

    Mouse monoclonal [SPM614] to Nucleolin

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey, OrangutanDoes not react with: Mouse, Rat, Cow

  • Immunogen

    Recombinant full length protein corresponding to Human Nucleolin aa 2-710.
    Sequence:

    VKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQK KGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPA KAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDD DSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDD EEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDE DDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGT EPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGY VDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLP YKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQG TEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEET LQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGR AIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIV TDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEG GFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHK PQGKKTKFE


    Database link: P19338

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab233945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Incubate with primary antibody for 30 minutes at room temperature.

Target

  • Function

    Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

  • Sequence similarities

    Contains 4 RRM (RNA recognition motif) domains.

  • Post-translational
    modifications

    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.

  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

  • Database links

    • Alternative names

      • C23 antibody
      • FLJ45706 antibody
      • MS1116 antibody
      • NCL antibody
      • Nucl antibody
      • NUCL_HUMAN antibody
      • Nucleolin antibody
      • Protein C23 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [SPM614] (ab233945)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [SPM614] (ab233945)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for Nucleolin using ab233945 at 1 μg/ml in immunohistochemical analysis.

    References

    ab233945 has not yet been referenced specifically in any publications.

    Publishing research using ab233945? Please let us know so that we can cite the reference in this datasheet.

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