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    nucleolin-phospho-t76-antibody-epr8080-2-ab168363.pdf

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Tags & Cell Markers Subcellular Markers Nucleus Nucleolus
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RecombinantRabMAb

Recombinant Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

  • Datasheet
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Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
  • Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
  • Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76)
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

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Carrier Free

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Overview

  • Product name

    Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
    See all Nucleolin primary antibodies
  • Description

    Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T cell lysates; Human tonsil tissue and urinary bladder transitional cell carcinoma tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8080-2
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nucleolus
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Alternative Versions

    • Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Nucleolin protein (His tag) (ab241527)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab168363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Application notes
Is unsuitable for ICC.

Target

  • Function

    Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
  • Sequence similarities

    Contains 4 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P19338 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4691 Human
    • Entrez Gene: 17975 Mouse
    • Entrez Gene: 25135 Rat
    • Omim: 164035 Human
    • SwissProt: P19338 Human
    • SwissProt: P09405 Mouse
    • SwissProt: P13383 Rat
    • Unigene: 79110 Human
    • Unigene: 154378 Mouse
    • Unigene: 474153 Mouse
    • Unigene: 144561 Rat
    see all
  • Alternative names

    • C23 antibody
    • FLJ45706 antibody
    • MS1116 antibody
    • NCL antibody
    • Nucl antibody
    • NUCL_HUMAN antibody
    • Nucleolin antibody
    • Protein C23 antibody
    see all

Images

  • Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
    Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
    All lanes : Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363) at 1/1000 dilution

    Lane 1 : 293T cell lysates, untreated
    Lane 2 : 293T cell lysates, treated with Lambda Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 77 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

    ab168363 showing +ve staining in Human ovarian carcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

    ab168363 showing +ve staining in Human kidney carcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

    ab168363 showing +ve staining in Human breast carcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

    ab168363 showing +ve staining in Human endometrial carcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Nucleolin with ab168363 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

    Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional cell carcinoma tissue labeling Nucleolin with ab168363 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
    Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
    Western blot analysis on immunoprecipitation pellet from 293 cell lysate using anti Phospho Nucleolin (pT76) ab158363 at 1/10 dilution.
  • Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
    Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab168363? Please let us know so that we can cite the reference in this datasheet.

ab168363 has been referenced in 1 publication.

  • Huang F  et al. Phosphorylation of nucleolin is indispensable to its involvement in the proliferation and migration of non-small cell lung cancer cells. Oncol Rep 41:590-598 (2019). PubMed: 30320343

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