Recombinant Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
![Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-166651-anti-nucleolin-phospho-t76-antibody-epr8080-2-western-blot.jpg "Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76)
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
See all Nucleolin primary antibodies -
Description
Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T cell lysates; Human tonsil tissue and urinary bladder transitional cell carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8080-2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
IP
1/10 - 1/100. |
Target
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Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Post-translational
modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 4691 Human
- Entrez Gene: 17975 Mouse
- Entrez Gene: 25135 Rat
- Omim: 164035 Human
- SwissProt: P19338 Human
- SwissProt: P09405 Mouse
- SwissProt: P13383 Rat
- Unigene: 79110 Human
see all -
Alternative names
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
Images
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Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)All lanes : Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363) at 1/1000 dilution
Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 77 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-168964-anti-nucleolin-phospho-t76-antibody-epr8080-2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)ab168363 showing +ve staining in Human ovarian carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-168963-anti-nucleolin-phospho-t76-antibody-epr8080-2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)ab168363 showing +ve staining in Human kidney carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-168962-anti-nucleolin-phospho-t76-antibody-epr8080-2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)ab168363 showing +ve staining in Human breast carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-168961-anti-nucleolin-phospho-t76-antibody-epr8080-2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)ab168363 showing +ve staining in Human endometrial carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-166650-anti-nucleolin-phospho-t76-antibody-epr8080-2-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Nucleolin with ab168363 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-166649-anti-nucleolin-phospho-t76-antibody-epr8080-2-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional cell carcinoma tissue labeling Nucleolin with ab168363 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-166648-anti-nucleolin-phospho-t76-antibody-epr8080-2-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)Western blot analysis on immunoprecipitation pellet from 293 cell lysate using anti Phospho Nucleolin (pT76) ab158363 at 1/10 dilution. -
![Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-9-benefits-of-recombinant-antibodies.png)
Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab168363 has been referenced in 1 publication.
- Huang F et al. Phosphorylation of nucleolin is indispensable to its involvement in the proliferation and migration of non-small cell lung cancer cells. Oncol Rep 41:590-598 (2019). PubMed: 30320343
