Recombinant Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76)
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
See all Nucleolin primary antibodies -
Description
Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76) -
Host species
Rabbit -
Specificity
ab168363 only detects Nucleolin when phosphorylated at threonine 76. -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Nucleolin.
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Positive control
- 293T cell lysates; Human tonsil tissue and urinary bladder transitional cell carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8080-2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab168363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Predicted molecular weight: 77 kDa. | |
IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
IP | 1/10 - 1/100. |
Target
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Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Post-translational
modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 4691 Human
- Entrez Gene: 17975 Mouse
- Entrez Gene: 25135 Rat
- Omim: 164035 Human
- SwissProt: P19338 Human
- SwissProt: P09405 Mouse
- SwissProt: P13383 Rat
- Unigene: 79110 Human
see all -
Alternative names
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
Images
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All lanes : Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363) at 1/1000 dilution
Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 77 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
ab168363 showing +ve staining in Human ovarian carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
ab168363 showing +ve staining in Human kidney carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
ab168363 showing +ve staining in Human breast carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
ab168363 showing +ve staining in Human endometrial carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Nucleolin with ab168363 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional cell carcinoma tissue labeling Nucleolin with ab168363 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from 293 cell lysate using anti Phospho Nucleolin (pT76) ab158363 at 1/10 dilution.
Protocols
Datasheets and documents
References (1)
ab168363 has been referenced in 1 publication.
- Huang F et al. Phosphorylation of nucleolin is indispensable to its involvement in the proliferation and migration of non-small cell lung cancer cells. Oncol Rep 41:590-598 (2019). PubMed: 30320343