Recombinant
Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Nucleolin (phospho T76) antibody [EPR8080-2]
See all Nucleolin primary antibodies -
DescriptionRabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76)
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Host speciesRabbit
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Specificityab168363 only detects Nucleolin when phosphorylated at threonine 76.
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Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic phosphopeptide, corresponding to residues surrounding threonine 76 of Human Nucleolin (UniProt: P19338).
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Positive control
- 293T cell lysates; Human tonsil tissue and urinary bladder transitional cell carcinoma tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR8080-2
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab168363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 77 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for ICC.
Target
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FunctionNucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
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Sequence similaritiesContains 4 RRM (RNA recognition motif) domains.
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Post-translational
modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localizationNucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
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Database links
- Entrez Gene: 4691 Human
- Entrez Gene: 17975 Mouse
- Entrez Gene: 25135 Rat
- Omim: 164035 Human
- SwissProt: P19338 Human
- SwissProt: P09405 Mouse
- SwissProt: P13383 Rat
- Unigene: 79110 Human
see all -
Alternative names
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-8-YCAR8080-Nucleolin-PhosphopT76-ovarian-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)ab168363 showing +ve staining in Human ovarian carcinoma. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-7-YCAR8080-Nucleolin-PhosphopT76-kidney-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)ab168363 showing +ve staining in Human kidney carcinoma. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-6-YCAR8080-Nucleolin-PhosphopT76-Infiltrating-duct-carcinoma-of-breast.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)ab168363 showing +ve staining in Human breast carcinoma. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/ab168363-5-YCAR8080-Nucleolin-PhosphopT76-Adenocarcinoma-of-endometrium.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)ab168363 showing +ve staining in Human endometrial carcinoma. -
![Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/Nucleolin-Primary-antibodies-ab168363-1.jpg)
Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)All lanes : Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363) at 1/1000 dilution
Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 77 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/Nucleolin-Primary-antibodies-ab168363-2.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Nucleolin with ab168363 at 1/50 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/Nucleolin-Primary-antibodies-ab168363-3.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional cell carcinoma tissue labeling Nucleolin with ab168363 at 1/50 dilution. -
![Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)](https://www.abcam.com/ps/products/168/ab168363/Images/Nucleolin-Primary-antibodies-ab168363-4.jpg)
Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)Western blot analysis on immunoprecipitation pellet from 293 cell lysate using anti Phospho Nucleolin (pT76) ab158363 at 1/10 dilution.
Datasheets and documents
References
ab168363 has not yet been referenced specifically in any publications.
