Name: Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
Brand: RabMAb
SKU: ab168363

Product name

Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
See all Nucleolin primary antibodies
Description

Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76)
Host species

Rabbit
Specificity

ab168363 only detects Nucleolin when phosphorylated at threonine 76.
Tested applications

Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic phosphopeptide, corresponding to residues surrounding threonine 76 of Human Nucleolin (UniProt: P19338).
Positive control
- 293T cell lysates; Human tonsil tissue and urinary bladder transitional cell carcinoma tissue.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8080-2
Isotype

IgG
Research areas

Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- 293T whole cell lysate (ab95494)

Our Abpromise guarantee covers the use of ab168363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP		1/10 - 1/100.

Application notes

Is unsuitable for ICC.

Function

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Sequence similarities

Contains 4 RRM (RNA recognition motif) domains.
Post-translational
modifications

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
Cellular localization

Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Target information above from: UniProt accession P19338 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4691 Human
- Entrez Gene: 17975 Mouse
- Entrez Gene: 25135 Rat
- Omim: 164035 Human
- SwissProt: P19338 Human
- SwissProt: P09405 Mouse
- SwissProt: P13383 Rat
- Unigene: 79110 Human
- Unigene: 154378 Mouse
- Unigene: 474153 Mouse
- Unigene: 144561 Rat
see all
Alternative names
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
- NCL antibody
- Nucl antibody
- NUCL_HUMAN antibody
- Nucleolin antibody
- Protein C23 antibody
see all

Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

All lanes : Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363) at 1/1000 dilution

Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with Lambda Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 77 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

ab168363 showing +ve staining in Human ovarian carcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

ab168363 showing +ve staining in Human kidney carcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

ab168363 showing +ve staining in Human breast carcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

ab168363 showing +ve staining in Human endometrial carcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Nucleolin with ab168363 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional cell carcinoma tissue labeling Nucleolin with ab168363 at 1/50 dilution.
Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

Western blot analysis on immunoprecipitation pellet from 293 cell lysate using anti Phospho Nucleolin (pT76) ab158363 at 1/10 dilution.

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Datasheet

ab168363 has not yet been referenced specifically in any publications.

Publishing research using ab168363? Please let us know so that we can cite the reference in this datasheet.

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Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

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