Key features and details
- Rabbit polyclonal to Nucleolin (phospho T84)
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nucleolin (phospho T84) antibody
See all Nucleolin primary antibodies
DescriptionRabbit polyclonal to Nucleolin (phospho T84)
SpecificityReacts with Nucleolin when phoshosphorylated at threonine 84. Cross reacts with phoshothreonine 106 (pT106). Does not cross react with phoshothreonine 76 & 92 (pT76 and pT92). The detection of Phospho nucleolin can be done during mitosis or in G2 phase, there is no signal in G1S phase.
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human Nucleolin (phospho T84).
Database link: P19338
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituents: 1.21% Tris, 0.75% Glycine
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab196338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/2000. Predicted molecular weight: 77 kDa.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||1/200 - 1/1000.|
FunctionNucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Sequence similaritiesContains 4 RRM (RNA recognition motif) domains.
modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
Cellular localizationNucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
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ab196338 has not yet been referenced specifically in any publications.