Reacts with Nucleolin when phoshosphorylated at threonine 84. Cross reacts with phoshothreonine 106 (pT106). Does not cross react with phoshothreonine 76 & 92 (pT76 and pT92). The detection of Phospho nucleolin can be done during mitosis or in G2 phase, there is no signal in G1S phase.
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Contains 4 RRM (RNA recognition motif) domains.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.