Recombinant
RabMAb

Recombinant Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8080] to Nucleolin (phospho T84)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Nucleolin (phospho T84) antibody [EPR8080]
    See all Nucleolin primary antibodies

  • Description

    Rabbit monoclonal [EPR8080] to Nucleolin (phospho T84)

  • Host species

    Rabbit

  • Specificity

    ab155977 only detects Nucleolin phosphorylated at threonine 84.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Nucleolin (phospho T84). The exact sequence is proprietary.
    Database link: P19338

  • Positive control

    • 293T cell lysate; ovarian carcinoma and tonsil tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab155977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

    • Sequence similarities

      Contains 4 RRM (RNA recognition motif) domains.

    • Post-translational
      modifications

      Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.

    • Cellular localization

      Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt

    • Database links

      • Alternative names

        • C23 antibody
        • FLJ45706 antibody
        • MS1116 antibody
        • NCL antibody
        • Nucl antibody
        • NUCL_HUMAN antibody
        • Nucleolin antibody
        • Protein C23 antibody
        see all

      Images

      • Western blot - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
        Western blot - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
        All lanes : Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977) at 1/1000 dilution

        Lane 1 : 293T cell lysate, untreated
        Lane 2 : 293T cell lysate treated with Lambda Phosphatase

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 77 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

        Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue, labeling Nucleolin using ab155977 at a 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

        Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Nucleolin using ab155977 at a 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References (0)

      Publishing research using ab155977? Please let us know so that we can cite the reference in this datasheet.

      ab155977 has not yet been referenced specifically in any publications.

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