RecombinantRabMAb

Recombinant Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

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Western blot - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
  • Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8080] to Nucleolin (phospho T84)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Carrier Free

Overview

  • Product name

    Anti-Nucleolin (phospho T84) antibody [EPR8080]
    See all Nucleolin primary antibodies

  • Description

    Rabbit monoclonal [EPR8080] to Nucleolin (phospho T84)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human esophagus tissue WB: K-562 treated with 100nM Calyculin A for 30 minutes whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100

Target

  • Function

    Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

  • Sequence similarities

    Contains 4 RRM (RNA recognition motif) domains.

  • Post-translational
    modifications

    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.

  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

  • Database links

    • Alternative names

      • C23 antibody
      • FLJ45706 antibody
      • MS1116 antibody
      • NCL antibody
      • Nucl antibody
      • NUCL_HUMAN antibody
      • Nucleolin antibody
      • Protein C23 antibody
      see all

    Images

    • Western blot - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
      Western blot - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
      All lanes : Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977) at 1/5000 dilution (Purified)

      Lane 1 : Untreated K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
      Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate
      Lane 3 : K-562 (Human chronic myelogenous leukemia lymphoblast) treated with 100nM Calyculin A for 30 minutes, then the membrane treated with Lambda Phosphatase for 1 hour

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 77 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue sections labeling Nucleolin with purified ab155977 at 1/5000 dilution (0.38 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

      Nuclear staining on human esophagus without alkaline phosphatase treatment (image A). No staining on human esophagus with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    • Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
      Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

    References (0)

    Publishing research using ab155977? Please let us know so that we can cite the reference in this datasheet.

    ab155977 has not yet been referenced specifically in any publications.

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