Phospho anti-Nucleolin (T84) antibody [EPR8080] (ab155977)

Product name

Anti-Nucleolin (phospho T84) antibody [EPR8080]
See all Nucleolin primary antibodies
Description

Rabbit monoclonal [EPR8080] to Nucleolin (phospho T84)
Host species

Rabbit
Specificity

ab155977 only detects Nucleolin phosphorylated at threonine 84.
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human Nucleolin (phospho T84). The exact sequence is proprietary.
Database link: P19338
Positive control
- 293T cell lysate; ovarian carcinoma and tonsil tissues.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8080
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab155977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt,ICC/IF or IP.

Function

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Sequence similarities

Contains 4 RRM (RNA recognition motif) domains.
Post-translational
modifications

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
Cellular localization

Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Target information above from: UniProt accession P19338 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4691 Human
- Omim: 164035 Human
- SwissProt: P19338 Human
- Unigene: 79110 Human
Alternative names
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
- NCL antibody
- Nucl antibody
- NUCL_HUMAN antibody
- Nucleolin antibody
- Protein C23 antibody
see all

Western blot - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

All lanes : Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977) at 1/1000 dilution

Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate treated with Lambda Phosphatase

Lysates/proteins at 10 µg per lane.

Predicted band size: 77 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue, labeling Nucleolin using ab155977 at a 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Nucleolin using ab155977 at a 1/50 dilution.

ab155977 has not yet been referenced specifically in any publications.

Publishing research using ab155977? Please let us know so that we can cite the reference in this datasheet.

Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)