Product nameAnti-Nucleophosmin antibody
See all Nucleophosmin primary antibodies
DescriptionRabbit polyclonal to Nucleophosmin
Tested applicationsSuitable for: IHC-P, IP, WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
- For western blotting, HepG2 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Purification notesThis antibody was purified by chromatography.
Our Abpromise guarantee covers the use of ab24412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|IP||Use at an assay dependent concentration. PubMed: 20053746|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. in 5% skim milk/PBS buffer. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionInvolved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).
Involvement in diseaseA chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated.
A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA.
A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1.
Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.
Sequence similaritiesBelongs to the nucleoplasmin family.
modificationsAcetylated at C-terminal lysine residues, thereby increasing affinity to histones.
Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
Sumoylated by ARF.
Cellular localizationNucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.
- Information by UniProt
- B23 antibody
- MGC104254 antibody
- NO38 antibody
Anti-Nucleophosmin antibody (ab24412) at 1 µg/ml + HepG2 cell lysate (10ug).
HRP conjugated Anti rabbit IgG at 1/50,000 - 1/100,000.
Predicted band size: 33 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Nucleophosmin antibody (ab24412) used at a concentration of 4-8 µg/ml. Human lung was used, and arrows point to alveolar cells (cells with positive label). X400 magnification.
All lanes : Anti-Nucleophosmin antibody (ab24412) at 1.25 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate with blocking peptide at 2 µg/ml
Lysates/proteins at 25 µg per lane.
Predicted band size: 33 kDa
This product has been referenced in:
- Huang M et al. Autophagy mediates proteolysis of NPM1 and HEXIM1 and sensitivity to BET inhibition in AML cells. Oncotarget 7:74917-74930 (2016). WB ; Human . Read more (PubMed: 27732946) »
- Ren Z et al. Phosphorylated STAT5 regulates p53 expression via BRCA1/BARD1-NPM1 and MDM2. Cell Death Dis 7:e2560 (2016). Read more (PubMed: 28005077) »