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Full length native protein (purified) (Rat).
Our Abpromise guarantee covers the use of ab10530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 33 kDa).|
|ELISA||Use at an assay dependent concentration.|
Western blot image of ab10530 staining whole cell lysate of U2OS cells. The gel was blocked with 5% milk for 1 hour at 21°C. The primary antibody was diluted to 2 µg/ml and incubated for 12 hours at 4°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.
Cell line lysate separated on SDS-PAGE, probed with ab10530 (0.5 μg/mL)
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells staining Nucleophosmin using ab10530 (dilution 1/500). Cells were fixed and permeabilized with methanol followed by methanol:acetone. Developed using Goat Anti-Mouse IgG, Cy3™ conjugate and counterstained with DAPI (blue) to stain nuclei.
ab10530 staining Nucleophosmin in immortalized Human trabecular meshwork cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% BSA for 45 minutes at room temperature. The primary antibody was diluted 1/1000 in PBS/0.3% BSA and incubated with the sample for 2 hours. The secondary antibody was Cy3®-conjugated Donkey anti-Mouse polyclonal, diluted 1/1000.
ab10530 at 1/250 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked with serum and incubated with the antibody for 30 minutes at 22°C. An HRP conjugated goat anti-mouse antibody was used as the secondary.
ab10530 staining Nucleophosmin in Mouse cystic kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2500 in blocking buffer) for 30 minutes at 22°C. A HRP-conjugated Goat anti-mouse polyclonal (1/400) was used as the secondary antibody.
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