Recombinant
RabMAb

Recombinant Anti-Nucleophosmin antibody [SP236] (ab183340)

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Overview

  • Product name

    Anti-Nucleophosmin antibody [SP236]
    See all Nucleophosmin primary antibodies

  • Description

    Rabbit monoclonal [SP236] to Nucleophosmin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow

  • Immunogen

    Synthetic peptide within Human Nucleophosmin aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P06748

  • Positive control

    • IHC-P: Human tonsil, Human skin, Mouse colon, and Rat spleen tissue; FC: HeLa and 3T3-L1 cells; ICC: 3T3-L1, HeLa and PC-12 cells.

  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP236

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/400. Predicted molecular weight: 33 kDa.
Flow Cyt 1/200 - 1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/100.

Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Function

    Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).

  • Involvement in disease

    A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated.
    A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA.
    A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1.
    Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.

  • Sequence similarities

    Belongs to the nucleoplasmin family.

  • Post-translational
    modifications

    Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.
    ADP-ribosylated.
    Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
    Sumoylated by ARF.

  • Cellular localization

    Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • B23 antibody
    • MGC104254 antibody
    • NO38 antibody
    • NPM antibody
    • NPM_HUMAN antibody
    • NPM1 antibody
    • Nucleolar phosphoprotein B23 antibody
    • Nucleolar protein NO38 antibody
    • Nucleophosmin (nucleolar phosphoprotein B23 numatrin) antibody
    • Nucleophosmin antibody
    • nucleophosmin nucleoplasmin family member 1 antibody
    • Nucleophosmin/nucleoplasmin family member 1 antibody
    • Numatrin antibody
    • OTTHUMP00000161024 antibody
    • OTTHUMP00000161025 antibody
    • OTTHUMP00000223397 antibody
    • OTTHUMP00000223398 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling Nucleophosmin with ab183340 at 1/100 dilution (2.51 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling Nucleophosmin with ab183340 at 1/100 dilution (2.51 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Nucleophosmin with ab183340 at 1/100 dilution (2.51 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] (ab183340)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Nucleophosmin with purified ab183340 at 1/100 (2.5µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Western blot - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Anti-Nucleophosmin antibody [SP236] (ab183340) at 1/400 dilution + NIH3T3 cell lysate

    Predicted band size: 33 kDa

  • Flow Cytometry - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Flow Cytometry - Anti-Nucleophosmin antibody [SP236] (ab183340)

    Flow cytometry analysis of 3T3-L1 (Mouse embryonic fibroblast) labeling Nucleophosmin with purified ab183340 at 1/200 dilution (1.255 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] (ab183340)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Nucleophosmin with ab183340 at a 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] (ab183340)

    Immunocytochemistry/ Immunofluorescence analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling Nucleophosmin with purified ab183340 at 1/100 (2.5µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] (ab183340)

    Immunocytochemistry/ Immunofluorescence analysis of 3T3-L1 (mouse embryonic fibroblast) cells labeling Nucleophosmin with purified ab183340 at 1/100 (2.5µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Flow Cytometry - Anti-Nucleophosmin antibody [SP236] (ab183340)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Nucleophosmin with purified ab183340 at 1/200 dilution (1.255 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

  • Flow Cytometry - Anti-Nucleophosmin antibody [SP236] (ab183340)
    Flow Cytometry - Anti-Nucleophosmin antibody [SP236] (ab183340)

    Flow cytometric analysis of Jurkat cells, labeling Nucleophosmin with ab183340 diluted 1/400 (green), compared with a negative control of anti-rabbit IgG (blue).

References

ab183340 has not yet been referenced specifically in any publications.

Publishing research using ab183340? Please let us know so that we can cite the reference in this datasheet.

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