Key features and details
- Rat monoclonal [2A] to Nucleoporin p62/NUP62
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
Product nameAnti-Nucleoporin p62/NUP62 antibody [2A]
See all Nucleoporin p62/NUP62 primary antibodies
DescriptionRat monoclonal [2A] to Nucleoporin p62/NUP62
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human, African green monkey
Does not react with: Mouse
Recombinant fragment (His-tag) corresponding to Human Nucleoporin p62/NUP62 aa 1-300. (N terminal proprietary tag).
MSGFNFGGTGAPTGGFTFGTAKTATTTPATGFSFSTSGTGGFNFGAPFQP ATSTPSTGLFSLATQTPATQTTGFTFGTATLASGGTGFSLGIGASKLNLS NTAATPAMANPSGFGLGSSNLTNAISSTVTSSQGTAPTGFVFGPSTTSVA PATTSGGFSFTGGSTAQPSGFNIGSAGNSAQPTAPATLPFTPATPAATTA GATQPAAPTPTATITSTGPSLFASIATAPTSSATTGLSLCTPVTTAGAPT AGTQGFSLKAPGAASGTSTTTSTAATATATTTSSSSTTGFALNLKPLAPA
Database link: P37198
Epitopeaa 1-179 (FG-repeat region)
- HeLa cells and Cos cells.
This product was previously labelled as Nucleoporin p62
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Concentration information loading...
Purification notesab188413 was purified from serum-free culture medium of the hybridoma.
Light chain typekappa
Our Abpromise guarantee covers the use of ab188413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).|
RelevanceThe nuclear pore complex is a structure that extends across the nuclear envelope and regulates the flow of macromolecules between the cytoplasm and the nucleus. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. Nup 62 is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. There are multiple transcript variants of this gene, however, they encode a single protein isoform. This protein undergoes post-translational modifications. It contains about 10 N-acetylglucosamine side chain sites.
Cellular localizationNucleus; nuclear pore complex. Cytoplasm; cytoskeleton; spindle pole. Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle.
- 62 kDa nucleoporin antibody
- DKFZp547L134 antibody
- FLJ20822 antibody
Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413) at 1/500 dilution + HeLa nuclear membrane lysate
Alkaline phosphatase-conjugated anti-rat IgG
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Immunofluorescent analysis of HeLa cells labeling Nucleoporin p62/NUP62 with ab188413 at 1/400 dilution, followed by Alexa Fluor®488-conjugated goat anti-rat IgG secondary antibody at 1/500 dilution. HeLa cells were fixed with 3.7% formaldehyde and
permeabilized with 0.5% Triton X-100.
Immunofluorescent analysis of HeLa cells, labeling Nucleoporin p62/NUP62 with ab188413 at 1/400 dilution, followed by Alexa Fluor®488-conjugated goat anti-rat IgG secondary antibody at 1/500 dilution. HeLa cells were fixed with 3.7% formaldehyde and
permeabilized with 0.5% Triton X-100.
ab188413 has been referenced in 4 publications.
- Heusel M et al. A Global Screen for Assembly State Changes of the Mitotic Proteome by SEC-SWATH-MS. Cell Syst 10:133-155.e6 (2020). PubMed: 32027860
- Fišerová J et al. Nuclear pore protein TPR associates with lamin B1 and affects nuclear lamina organization and nuclear pore distribution. Cell Mol Life Sci 76:2199-2216 (2019). PubMed: 30762072
- Fišerová J et al. Chromatin organization at the nuclear periphery as revealed by image analysis of structured illumination microscopy data. J Cell Sci 130:2066-2077 (2017). PubMed: 28476938
- Linder MI et al. Mitotic Disassembly of Nuclear Pore Complexes Involves CDK1- and PLK1-Mediated Phosphorylation of Key Interconnecting Nucleoporins. Dev Cell 43:141-156.e7 (2017). PubMed: 29065306