Overview

  • Product name

    Anti-Nucleoporin p62/NUP62 antibody
    See all Nucleoporin p62/NUP62 primary antibodies
  • Description

    Rabbit polyclonal to Nucleoporin p62/NUP62
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Nucleoporin p62/NUP62 aa 75-125. The exact sequence is proprietary.
    Database link: P37198

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
  • General notes

     This product was previously labelled as Nucleoporin p62

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 53 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    The nuclear pore complex is a structure that extends across the nuclear envelope and regulates the flow of macromolecules between the cytoplasm and the nucleus. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. Nup 62 is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. There are multiple transcript variants of this gene, however, they encode a single protein isoform. This protein undergoes post-translational modifications. It contains about 10 N-acetylglucosamine side chain sites.
  • Cellular localization

    Nucleus; nuclear pore complex. Cytoplasm; cytoskeleton; spindle pole. Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle.
  • Database links

  • Alternative names

    • 62 kDa nucleoporin antibody
    • DKFZp547L134 antibody
    • FLJ20822 antibody
    • FLJ43869 antibody
    • IBSN antibody
    • MGC841 antibody
    • Nuclear pore glycoprotein p62 antibody
    • nucleoporin 62kDa antibody
    • Nucleoporin Nup62 antibody
    • nucleoporin p62 antibody
    • nucleoporin p62KD antibody
    • NUP62 antibody
    • NUP62 protein antibody
    • p62 antibody
    • SNDI antibody
    see all

Images

  • All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab226386) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 53 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • Nucleoporin p62/NUP62 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226386 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226386 at 0.4 µg/ml.

    Lane 1: ab226386 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 30 seconds.

References

ab226386 has not yet been referenced specifically in any publications.

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