Key features and details
- Rabbit polyclonal to Nucleoporin p62/NUP62
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-Nucleoporin p62/NUP62 antibody
See all Nucleoporin p62/NUP62 primary antibodies
DescriptionRabbit polyclonal to Nucleoporin p62/NUP62
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human, Pig
Recombinant fragment (His-T7-tag) corresponding to Human Nucleoporin p62/NUP62 aa 186-432. (Expressed in E.coli).
ATLPFTPATPAATTAGATQPAAPTPTATITSTGPSLFASIATAPTSSATT GLSLCTPVTTAGAPTAGTQGFSLKAPGAASGTSTTTSTAATATATTTSSS STTGFALNLKPLAPAGIPSNTAAAVTAPPGPGAAAGAAASSAMTYAQLES LINKWSLELEDQERHFLQQATQVNAWDRTLIENGEKITSLHREVEKVKLD QKRLDQELDFILSQQKELEDLLSPLEELVKEQSGTIYLQHADEEREK
Database link: P37198
- ICC/IF: HepG2 cells. IHC-P; Human testis tissue. WB: Pig liver lysate; HepG2, Raji and A549 cell lysates; Recombinant human Nucleoporin p62/NUP62 protein.
This product was previously labelled as Nucleoporin p62
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232816 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 53 kDa.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
RelevanceThe nuclear pore complex is a structure that extends across the nuclear envelope and regulates the flow of macromolecules between the cytoplasm and the nucleus. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. Nup 62 is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. There are multiple transcript variants of this gene, however, they encode a single protein isoform. This protein undergoes post-translational modifications. It contains about 10 N-acetylglucosamine side chain sites.
Cellular localizationNucleus; nuclear pore complex. Cytoplasm; cytoskeleton; spindle pole. Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle.
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Formalin-fixed, paraffin-embedded human testis tissue stained for Nucleoporin p62/NUP62 using ab232816 at 20 µg/ml in immunohistochemical analysis. DAB staining.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Nucleoporin p62/NUP62 (green) using ab232816 at 20 µg/ml in ICC/IF, followed by FITC staining.
Anti-Nucleoporin p62/NUP62 antibody (ab232816) at 2 µg/ml + Recombinant human Nucleoporin p62/NUP62 protein
Predicted band size: 53 kDa
All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab232816) at 3 µg/ml
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 4 : Pig liver lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 53 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232816 has not yet been referenced specifically in any publications.