Anti-Nucleoporin p62/NUP62 antibody (ab95956)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Nucleoporin p62/NUP62 antibody
See all Nucleoporin p62/NUP62 primary antibodies -
Description
Rabbit polyclonal to Nucleoporin p62/NUP62 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Zebrafish
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Immunogen
Recombinant fragment corresponding to Human Nucleoporin p62/NUP62 aa 230-500.
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Positive control
- HepG2, MOLT4 and Raji whole cell lysates. Hela, 293T, A431, H1299 and HeLaS3 cells. NCIN87 xenograft.
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General notes
This product was previously labelled as Nucleoporin p62
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 53 kDa. | |
| IHC-P | 1/100 - 1/500. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Relevance
The nuclear pore complex is a structure that extends across the nuclear envelope and regulates the flow of macromolecules between the cytoplasm and the nucleus. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. Nup 62 is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. There are multiple transcript variants of this gene, however, they encode a single protein isoform. This protein undergoes post-translational modifications. It contains about 10 N-acetylglucosamine side chain sites. -
Cellular localization
Nucleus; nuclear pore complex. Cytoplasm; cytoskeleton; spindle pole. Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle. -
Database links
- Entrez Gene: 23636 Human
- Entrez Gene: 18226 Mouse
- Entrez Gene: 65274 Rat
- Omim: 605815 Human
- SwissProt: P37198 Human
- SwissProt: Q63850 Mouse
- SwissProt: P17955 Rat
- Unigene: 574492 Human
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Alternative names
- 62 kDa nucleoporin antibody
- DKFZp547L134 antibody
- FLJ20822 antibody
see all
Images
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Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab95956)All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab95956) at 1/5000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lane 3 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody (ab95956)ab95956, at a 1/200 dilution, staining Nucleoporin p62/NUP62 in paraformaldehyde fixed HeLa by Immunofluorescence.
Image on the right: merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody (ab95956)ab95956, at a 1/500 dilution, staining Nucleoporin p62/NUP62 in paraffin embedded NCIN87 xenograft.
Protocols
References
This product has been referenced in:
- Schaefer CP et al. Chronic morphine exposure potentiates p-glycoprotein trafficking from nuclear reservoirs in cortical rat brain microvessels. PLoS One 13:e0192340 (2018). Read more (PubMed: 29414996) »
