Anti-Nucleoporin p62/NUP62 antibody (ab96134)
Key features and details
- Rabbit polyclonal to Nucleoporin p62/NUP62
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Nucleoporin p62/NUP62 antibody
See all Nucleoporin p62/NUP62 primary antibodies -
Description
Rabbit polyclonal to Nucleoporin p62/NUP62 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cow, Zebrafish, Rhesus monkey
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Immunogen
Recombinant fragment corresponding to Human Nucleoporin p62/NUP62 aa 294-502.
Database link: NP_057637 -
Positive control
- WB: A431, H1299, PC-12, Rat2, Neuro-2a, GL261, C8D30, NIH/3T3, BCL-1, RAW 264.7 and C2C12 whole cell lysate. ICC/IF: A431 cells. IHC-P: Mouse fore brain tissue.
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General notes
This product was previously labelled as Nucleoporin p62
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab96134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 53 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Relevance
The nuclear pore complex is a structure that extends across the nuclear envelope and regulates the flow of macromolecules between the cytoplasm and the nucleus. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. Nup 62 is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. There are multiple transcript variants of this gene, however, they encode a single protein isoform. This protein undergoes post-translational modifications. It contains about 10 N-acetylglucosamine side chain sites. -
Cellular localization
Nucleus; nuclear pore complex. Cytoplasm; cytoskeleton; spindle pole. Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle. -
Database links
- Entrez Gene: 23636 Human
- Entrez Gene: 65274 Rat
- Entrez Gene: 100426308 Rhesus monkey
- Omim: 605815 Human
- SwissProt: P37198 Human
- SwissProt: P17955 Rat
- Unigene: 574492 Human
- Unigene: 54450 Rat
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Alternative names
- 62 kDa nucleoporin antibody
- DKFZp547L134 antibody
- FLJ20822 antibody
see all
Images
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Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134)All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 5 : BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 53 kDa10% SDS-PAGE.
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Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody (ab96134)ab96134, at a 1/200 dilution, staining Nucleoporin p62/NUP62 in paraformaldehyde fixed A431 by Immunofluorescence analysis.
Lower image: merged with DNA probe. -
Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134)All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (Rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 53 kDa10% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody (ab96134)Paraffin-embedded mouse fore brain tissue stained for Nucleoporin p62/NUP62 using ab96134 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134)All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody (ab96134)Immunofluorescence analysis of A431 cells, staining Nucleoporin p62/NUP62 with ab96134 at 1/200 dilution, demonstrating punctate nuclear staining.
Protocols
References (7)
ab96134 has been referenced in 7 publications.
- Qiao J et al. Inhibition of HIF-1a restrains fracture healing via regulation of autophagy in a rat model. Exp Ther Med 17:1884-1890 (2019). PubMed: 30783464
- Clift D et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell 171:1692-1706.e18 (2017). PubMed: 29153837
- Ouyang F et al. HMGB1 induces apoptosis and EMT in association with increased autophagy following H/R injury in cardiomyocytes. Int J Mol Med 37:679-89 (2016). WB . PubMed: 26847839
- Lund PJ et al. Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol 197:3086-3098 (2016). Human . PubMed: 27655845
- Guan JJ et al. DRAM1 regulates apoptosis through increasing protein levels and lysosomal localization of BAX. Cell Death Dis 6:e1624 (2015). PubMed: 25633293
- Tojo Y et al. Hypoxia Signaling Cascade for Erythropoietin Production in Hepatocytes. Mol Cell Biol 35:2658-72 (2015). Mouse . PubMed: 26012551
- Isnard A et al. Impact of Leishmania infection on host macrophage nuclear physiology and nucleopore complex integrity. PLoS Pathog 11:e1004776 (2015). WB . PubMed: 25826301
