Key features and details
- Rabbit polyclonal to Nucleoside-diphosphate kinase
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nucleoside-diphosphate kinase antibody
See all Nucleoside-diphosphate kinase primary antibodies
DescriptionRabbit polyclonal to Nucleoside-diphosphate kinase
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Recombinant fragment corresponding to Human Nucleoside-diphosphate kinase aa 37-196.
LLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQK NKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGK SSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFD EVVQIFDKEG
Database link: P30085-1
- WB: HEK-293T and HeLa whole cell lysates. IHC-P: Human colon cancer tissue.
This product was previously labelled as UMP/CMP kinase
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 22 kDa.|
FunctionCatalyzes specific phosphoryl transfer from ATP to UMP and CMP.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the adenylate kinase family.
Cellular localizationNucleus. Cytoplasm. Mainly nuclear.
- Information by UniProt
- CMK antibody
- CMPK 1 antibody
- CMPK antibody
Paraffin-embedded human colon cancer tissue stained for Nucleoside-diphosphate kinase using ab225940 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-Nucleoside-diphosphate kinase antibody (ab225940) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225940 has not yet been referenced specifically in any publications.