Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)

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Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
  • Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
  • Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
  • Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14325-50] to Nucleoside triphosphate phosphohydrolase - N-terminal
  • Suitable for: WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal
    See all Nucleoside triphosphate phosphohydrolase primary antibodies

  • Description

    Rabbit monoclonal [EPR14325-50] to Nucleoside triphosphate phosphohydrolase - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Nucleoside triphosphate phosphohydrolase aa 1-150. The exact sequence is proprietary.
    Database link: Q9BSD7

  • Positive control

    • WB: HEK293T, U87-MG, Jurkat, A549 cell lysates. ICC: U87-MG and A549 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14325-50

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ICC/IF 1/100.
IP 1/50.

Target

  • Relevance

    Nucleoside triphosphate phosphohydrolase (Chromosome 1 open reading frame 57) belongs to the UPF0334 family. The function of C1orf57 is unknown.

  • Database links

    • Alternative names

      • C1orf57 antibody
      • Chromosome 1 open reading frame 57 antibody
      • FLJ11383 antibody
      • HCR NTPase antibody
      • Human cancer related NTPase antibody
      • Hypothetical protein LOC84284 antibody
      • MGC13186 antibody
      • Novel DUF829 domain containing protein antibody
      • Nucleoside triphosphatase C1orf57 antibody
      • Nucleoside triphosphatase, cancer related antibody
      • Nucleoside triphosphate phosphohydrolase antibody
      • OTTHUMP00000035941 antibody
      • OTTHUMP00000035942 antibody
      • Probable UPF0334 kinase like protein C1orf57 antibody
      • RP4 659I19.2 antibody
      • RP4 678E16.2 antibody
      see all

    Images

    • Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
      Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
      All lanes : Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : NTPCR knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
      Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab182155 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab182155 Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal was shown to specifically react with Nucleoside triphosphate phosphohydrolase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266397 (knockout cell lysate ab258083) was used. Wild-type and Nucleoside triphosphate phosphohydrolase knockout samples were subjected to SDS-PAGE. ab182155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
      Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)

      Immunofluorescence analysis of A549 cells, staining Nucleoside triphosphate phosphohydrolase (green) with ab182155 at 1/100 dilution. An Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody. Nuclei were counterstained with DAPI (blue).

    • Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
      Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
      All lanes : Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155) at 1/20000 dilution

      Lane 1 : U87-MG cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
      Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)

      Immunofluorescence analysis of U87-MG cells, staining Nucleoside triphosphate phosphohydrolase (red) with ab182155 at 1/100 dilution. An Alexa Fluor®555-conjugated goat anti rabbit IgG was used as a secondary antibody. Nuclei were counterstained with DAPI (blue).

    • Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
      Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)

      Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate (lane 1) or negative control (lane 2), labeling Nucleoside triphosphate phosphohydrolase immunoprecipitated using ab182155 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)
      Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (ab182155)

    References (0)

    Publishing research using ab182155? Please let us know so that we can cite the reference in this datasheet.

    ab182155 has not yet been referenced specifically in any publications.

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