Recombinant
RabMAb

Recombinant Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

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Overview

  • Product name

    Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325]
    See all Nucleoside triphosphate phosphohydrolase primary antibodies

  • Description

    Rabbit monoclonal [EPR14325] to Nucleoside triphosphate phosphohydrolase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Nucleoside triphosphate phosphohydrolase aa 1-150. The exact sequence is proprietary.
    Database link: Q9BSD7

  • Positive control

    • U87-MG and Jurkat cell lysates; Jurkat cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14325

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/230.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IP 1/20.

Target

  • Relevance

    Nucleoside triphosphate phosphohydrolase (Chromosome 1 open reading frame 57) belongs to the UPF0334 family. The function of C1orf57 is unknown.

  • Database links

    • Alternative names

      • C1orf57 antibody
      • Chromosome 1 open reading frame 57 antibody
      • FLJ11383 antibody
      • HCR NTPase antibody
      • Human cancer related NTPase antibody
      • Hypothetical protein LOC84284 antibody
      • MGC13186 antibody
      • Novel DUF829 domain containing protein antibody
      • Nucleoside triphosphatase C1orf57 antibody
      • Nucleoside triphosphatase, cancer related antibody
      • Nucleoside triphosphate phosphohydrolase antibody
      • OTTHUMP00000035941 antibody
      • OTTHUMP00000035942 antibody
      • Probable UPF0334 kinase like protein C1orf57 antibody
      • RP4 659I19.2 antibody
      • RP4 678E16.2 antibody
      see all

    Images

    • Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
      Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
      All lanes : Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154) at 1/2000 dilution

      Lane 1 : U87-MG cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 21 kDa

    • Flow Cytometry - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
      Flow Cytometry - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

      Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling Nucleoside triphosphate phosphohydrolase with ab182154 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

    • Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
      Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

      Western blot analysis of immunoprecipitation pellet from U87-MG cell lysate immunoprecipitated using ab182154 at 1/20 dilution.
      Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    References

    ab182154 has not yet been referenced specifically in any publications.

    Publishing research using ab182154? Please let us know so that we can cite the reference in this datasheet.

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