Recombinant Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
![Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)](https://www.abcam.com/ps/products/182/ab182154/Images/ab182154-412761-anti-nucleoside-triphosphate-phosphohydrolase-antibody-epr14325-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14325] to Nucleoside triphosphate phosphohydrolase
- Suitable for: Flow Cyt, WB, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325]
See all Nucleoside triphosphate phosphohydrolase primary antibodies -
Description
Rabbit monoclonal [EPR14325] to Nucleoside triphosphate phosphohydrolase -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Nucleoside triphosphate phosphohydrolase aa 1-150. The exact sequence is proprietary.
Database link: Q9BSD7 -
Positive control
- WB: HEK-293T, U87-MG and Jurkat cell lysates. Flow Cyt: Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14325 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab182154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/230. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
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| WB | 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). | |
| IP | 1/20. |
Target
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Relevance
Nucleoside triphosphate phosphohydrolase (Chromosome 1 open reading frame 57) belongs to the UPF0334 family. The function of C1orf57 is unknown. -
Database links
- Entrez Gene: 84284 Human
- SwissProt: Q9BSD7 Human
- Unigene: 642715 Human
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Alternative names
- C1orf57 antibody
- Chromosome 1 open reading frame 57 antibody
- FLJ11383 antibody
see all
Images
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Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)All lanes : Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : NTPCR knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaLanes 1-4: Merged signal (red and green). Green - ab182154 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.
ab182154 Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] was shown to specifically react with Nucleoside triphosphate phosphohydrolase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266397 (knockout cell lysate ab258083) was used. Wild-type and Nucleoside triphosphate phosphohydrolase knockout samples were subjected to SDS-PAGE. ab182154 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)](https://www.abcam.com/ps/products/182/ab182154/Images/ab182154-216054-ab182154b.jpg)
Flow Cytometry - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling Nucleoside triphosphate phosphohydrolase with ab182154 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)](https://www.abcam.com/ps/products/182/ab182154/Images/ab182154-216056-ab182154.jpg)
Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)All lanes : Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154) at 1/2000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDa -
![Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)](https://www.abcam.com/ps/products/182/ab182154/Images/ab182154-216052-ab182154c.jpg)
Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)Western blot analysis of immunoprecipitation pellet from U87-MG cell lysate immunoprecipitated using ab182154 at 1/20 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)](https://www.abcam.com/ps/products/182/ab182154/Images/ab182154-5-benefits-of-recombinant-antibodies.png)
Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab182154 has not yet been referenced specifically in any publications.
