Recombinant Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)
![Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)](https://www.abcam.com/ps/products/250/ab250596/Images/ab250596-412786-anti-nucleoside-triphosphate-phosphohydrolase-antibody-epr14325-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14325] to Nucleoside triphosphate phosphohydrolase - BSA and Azide free
- Suitable for: IP, Flow Cyt, WB
- Reacts with: Human
Overview
-
Product name
Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free
See all Nucleoside triphosphate phosphohydrolase primary antibodies -
Description
Rabbit monoclonal [EPR14325] to Nucleoside triphosphate phosphohydrolase - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP HumanWB Human
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HEK-293T, U87-MG and Jurkat cell lysates. Flow Cyt: Jurkat cells.
-
General notes
Ab250596 is the carrier-free version of ab182154. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250596 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14325 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| IP |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
|
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Target
-
Relevance
Nucleoside triphosphate phosphohydrolase (Chromosome 1 open reading frame 57) belongs to the UPF0334 family. The function of C1orf57 is unknown. -
Database links
- Entrez Gene: 84284 Human
- SwissProt: Q9BSD7 Human
- Unigene: 642715 Human
-
Alternative names
- C1orf57 antibody
- Chromosome 1 open reading frame 57 antibody
- FLJ11383 antibody
see all
Images
-
Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)All lanes : Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : NTPCR knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaThis data was developed using ab182154, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab182154 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.
ab182154 Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] was shown to specifically react with Nucleoside triphosphate phosphohydrolase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266397 (knockout cell lysate ab258083) was used. Wild-type and Nucleoside triphosphate phosphohydrolase knockout samples were subjected to SDS-PAGE. ab182154 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Flow Cytometry - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)](https://www.abcam.com/ps/products/250/ab250596/Images/ab250596-2-anti-nucleoside-triphosphate-phosphohydrolase-antibody-epr14325-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)This data was developed using ab182154, the same antibody clone in a different buffer formulation.Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling Nucleoside triphosphate phosphohydrolase with ab182154 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. -
![Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)](https://www.abcam.com/ps/products/250/ab250596/Images/ab250596-405992-anti-nucleoside-triphosphate-phosphohydrolase-antibody-epr14325-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)All lanes : Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154) at 1/2000 dilution
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDaThis data was developed using ab182154, the same antibody clone in a different buffer formulation.
-
![Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)](https://www.abcam.com/ps/products/250/ab250596/Images/ab250596-1-anti-nucleoside-triphosphate-phosphohydrolase-antibody-epr14325-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)This data was developed using ab182154, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from U87-MG cell lysate immunoprecipitated using ab182154 at 1/20 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)](https://www.abcam.com/ps/products/250/ab250596/Images/ab250596-5-benefits-of-recombinant-antibodies.png)
Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab250596 has not yet been referenced specifically in any publications.