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    nucleostemin-antibody-ab70346.pdf

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Neuroscience Cell Type Marker Neural Stem Cell marker
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Anti-Nucleostemin antibody (ab70346)

  • Datasheet
Reviews (10) Submit a question References (11)

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Western blot - Anti-Nucleostemin antibody (ab70346)
  • Western blot - Anti-Nucleostemin antibody (ab70346)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)
  • Immunoprecipitation - Anti-Nucleostemin antibody (ab70346)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleostemin antibody (ab70346)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)

Key features and details

  • Rabbit polyclonal to Nucleostemin
  • Suitable for: ICC/IF, IHC-P, WB, IP
  • Reacts with: Mouse, Rat, Chicken, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Nucleostemin antibody [EPR3911(2)] (ab129185)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Nucleostemin antibody
    See all Nucleostemin primary antibodies
  • Description

    Rabbit polyclonal to Nucleostemin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human
    Predicted to work with: Rabbit, Horse, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human Nucleostemin (C terminal).
    Database link: Q9BVP2

  • Positive control

    • HeLa, 293T and mouse 3T3 whole cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab70346 was affinity purified using an epitope specific to Nucleostemin immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Stem Cells
    • Mesenchymal Stem Cells
    • Intracellular
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab70346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
Use a concentration of 1 µg/ml.
IHC-P (3)
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (4)
1/2000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IP (1)
Use at 1-2 µg/mg of lysate.
Notes
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IP
Use at 1-2 µg/mg of lysate.

Target

  • Function

    May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
  • Tissue specificity

    Increased levels in lung tissue in cancer patients.
  • Sequence similarities

    Belongs to the MMR1/HSR1 GTP-binding protein family.
    Contains 1 G (guanine nucleotide-binding) domain.
  • Domain

    The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.
    In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
  • Cellular localization

    Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.
  • Target information above from: UniProt accession Q9BVP2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 26354 Human
    • Entrez Gene: 30877 Mouse
    • Entrez Gene: 290556 Rat
    • Omim: 608011 Human
    • SwissProt: Q9BVP2 Human
    • SwissProt: Q8CI11 Mouse
    • SwissProt: Q811S9 Rat
    • Unigene: 313544 Human
    • Unigene: 88512 Mouse
    • Unigene: 4110 Rat
    see all
  • Alternative names

    • C77032 antibody
    • E2 induced gene 3 protein antibody
    • E2-induced gene 3 protein antibody
    • E2IG3 antibody
    • Estradiol induced nucleotide binding protein antibody
    • GNL3 antibody
    • GNL3_HUMAN antibody
    • Guanine nucleotide binding protein like 3 (nucleolar) antibody
    • Guanine nucleotide binding protein like 3 antibody
    • Guanine nucleotide-binding protein-like 3 antibody
    • MGC800 antibody
    • NNP47 antibody
    • Novel nucleolar protein 47 antibody
    • NS antibody
    • Nucleolar GTP binding protein 3 antibody
    • Nucleolar GTP-binding protein 3 antibody
    • Nucleostemin antibody
    • Putative nucleotide binding protein estradiol induced antibody
    see all

Images

  • Western blot - Anti-Nucleostemin antibody (ab70346)
    Western blot - Anti-Nucleostemin antibody (ab70346)
    All lanes : Anti-Nucleostemin antibody (ab70346) at 1/5000 dilution

    Lane 1 : HeLa Whole cell lysate
    Lane 2 : HEK293T Whole cell lysate
    Lane 3 : Jurkat Whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit Light Chain HRP Conjugate

    Predicted band size: 62 kDa



    Detection: Chemiluminescence with an exposure time of 10 seconds

  • Western blot - Anti-Nucleostemin antibody (ab70346)
    Western blot - Anti-Nucleostemin antibody (ab70346)
    All lanes : Anti-Nucleostemin antibody (ab70346) at 1/2000 dilution

    Lane 1 : TMCK-1 Whole cell lysate
    Lane 2 : NIH 3T3 :Whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit Light Chain HRP Conjugate

    Predicted band size: 62 kDa



    Detection: Chemiluminescence with an exposure time of 30 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded section of mouse CT26 colon carcinoma tissue labelling Nucleostemin with ab70346 at 1:200 (1µg/ml). Detection: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded section of human breast carcinoma tissue labelling Nucleostemin with ab70346 at 1:200 (1µg/ml). Detection: DAB.

  • Immunoprecipitation - Anti-Nucleostemin antibody (ab70346)
    Immunoprecipitation - Anti-Nucleostemin antibody (ab70346)

    Nucleostemin was immunoprecipitated from HEK-293T whole cell lysate with 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab70346 at 0.04 µg/ml dilution.

    Lane 1: ab70346 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleostemin antibody (ab70346)
    Immunocytochemistry/ Immunofluorescence - Anti-Nucleostemin antibody (ab70346)
    ICC/IF image of ab70346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70346, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom
    Immunohistochemistical detection of Nucleostemin with ab70346 on formaldehyde-fixed paraffin-embedded human colon sections. Antigen retrieval step: heat mediated in citric acid pH6 buffer. Blocking step: 1% BSA  for 10 mins @ rt°C. Primary antibody ab70346 incubated at 1/50 for 16 hours in TBS/BSA/azide. Secondary antibody: anti-rabbit IgG conjugated to biotin (1/200).  The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. Stem cell markers have been observed in cycling columnar cells at the crypt base (Nature 449, 1003-1007; 2007). The cells (nuclei of the simple columnar epithelium of the upper mucosa) immunoreactive for Nucleostemin in this image (green arrowheads) anatomically correspond to the same cells expected to be Lgr5 positivite. A red arrowhead indicates nucleolar positivity in one of many cell nuclei in the Lamina propria of the mucosa (this area is not known for stem cell posit

    See Abreview

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (11)

Publishing research using ab70346? Please let us know so that we can cite the reference in this datasheet.

ab70346 has been referenced in 11 publications.

  • Sukocheva OA  et al. Divergence of Intracellular Trafficking of Sphingosine Kinase 1 and Sphingosine-1-Phosphate Receptor 3 in MCF-7 Breast Cancer Cells and MCF-7-Derived Stem Cell-Enriched Mammospheres. Int J Mol Sci 22:N/A (2021). PubMed: 33919234
  • Zhang X  et al. Nucleostemin promotes hepatocellular carcinoma by regulating the function of STAT3. Exp Cell Res 387:111748 (2020). PubMed: 31785228
  • Ho TC  et al. PEDF-derived peptide promotes tendon regeneration through its mitogenic effect on tendon stem/progenitor cells. Stem Cell Res Ther 10:2 (2019). PubMed: 30606221
  • Francipane MG  et al. Establishment and Characterization of 5-Fluorouracil-Resistant Human Colorectal Cancer Stem-Like Cells: Tumor Dynamics under Selection Pressure. Int J Mol Sci 20:N/A (2019). PubMed: 31013771
  • Yildirim GD  et al. High nucleostemin expression has a favorable prognostic effect on gastric carcinomas Turk J Med Sci 48:484-490 (2018). PubMed: 29914242
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 10 Abreviews or Q&A

Western blot abreview for Anti-Nucleostemin antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Sep 12 2017

Western blot abreview for Anti-Nucleostemin antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10 % GEL, SDS)
Sample
Human Cell lysate - whole cell (HEK293, HELA, HCT116)
Specification
HEK293, HELA, HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Apr 24 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Nucleostemin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Human Cell (HEK293)
Specification
HEK293
Permeabilization
Yes - TRITON
Fixative
Methanol
Read More

Abcam user community

Verified customer

Submitted Apr 23 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Nucleostemin antibody

Excellent
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela Cells)
Specification
Hela Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - .25% Triton X-100 in PBS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Ms. Western,IP ChIP

Verified customer

Submitted Oct 22 2011

Western blot abreview for Anti-Nucleostemin antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Hela Cells)
Loading amount
30 µg
Specification
Hela Cells
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Oct 22 2011

Western blot abreview for Anti-Nucleostemin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse ES cells)
Loading amount
30 µg
Specification
Mouse ES cells
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Oct 22 2011

Immunoprecipitation abreview for Anti-Nucleostemin antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Hela Cells)
Total protein in input
200000 µg
Specification
Hela Cells
Immuno-precipitation step
Protein G
Read More

Abcam user community

Verified customer

Submitted Oct 21 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nucleostemin antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Read More

MR. Carl Hobbs

Verified customer

Submitted Feb 15 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nucleostemin antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (chick day 6 embryo liver and kidney)
Specification
chick day 6 embryo liver and kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Read More

MR. Carl Hobbs

Verified customer

Submitted Feb 15 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nucleostemin antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (adult rat brain)
Specification
adult rat brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Read More

MR. Carl Hobbs

Verified customer

Submitted Feb 15 2010

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