Anti-Nucleostemin antibody (ab70346)
Key features and details
- Rabbit polyclonal to Nucleostemin
- Suitable for: ICC/IF, IHC-P, WB, IP
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Nucleostemin antibody
See all Nucleostemin primary antibodies -
Description
Rabbit polyclonal to Nucleostemin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human
Predicted to work with: Rabbit, Horse, Dog, Chimpanzee
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Immunogen
Synthetic peptide corresponding to Human Nucleostemin (C terminal).
Database link: Q9BVP2 -
Positive control
- HeLa, 293T and mouse 3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab70346 was affinity purified using an epitope specific to Nucleostemin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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| IHC-P | (3) |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (4) |
1/2000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
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| IP | (1) |
Use at 1-2 µg/mg of lysate.
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| Notes |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
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IP
Use at 1-2 µg/mg of lysate. |
Target
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Function
May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis. -
Tissue specificity
Increased levels in lung tissue in cancer patients. -
Sequence similarities
Belongs to the MMR1/HSR1 GTP-binding protein family.
Contains 1 G (guanine nucleotide-binding) domain. -
Domain
The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.
In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern. -
Cellular localization
Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 26354 Human
- Entrez Gene: 30877 Mouse
- Entrez Gene: 290556 Rat
- Omim: 608011 Human
- SwissProt: Q9BVP2 Human
- SwissProt: Q8CI11 Mouse
- SwissProt: Q811S9 Rat
- Unigene: 313544 Human
see all -
Alternative names
- C77032 antibody
- E2 induced gene 3 protein antibody
- E2-induced gene 3 protein antibody
see all
Images
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Western blot - Anti-Nucleostemin antibody (ab70346)All lanes : Anti-Nucleostemin antibody (ab70346) at 1/5000 dilution
Lane 1 : HeLa Whole cell lysate
Lane 2 : HEK293T Whole cell lysate
Lane 3 : Jurkat Whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 62 kDaDetection: Chemiluminescence with an exposure time of 10 seconds
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Western blot - Anti-Nucleostemin antibody (ab70346)All lanes : Anti-Nucleostemin antibody (ab70346) at 1/2000 dilution
Lane 1 : TMCK-1 Whole cell lysate
Lane 2 : NIH 3T3 :Whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 62 kDaDetection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded section of mouse CT26 colon carcinoma tissue labelling Nucleostemin with ab70346 at 1:200 (1µg/ml). Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded section of human breast carcinoma tissue labelling Nucleostemin with ab70346 at 1:200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Nucleostemin antibody (ab70346)Nucleostemin was immunoprecipitated from HEK-293T whole cell lysate with 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab70346 at 0.04 µg/ml dilution.
Lane 1: ab70346 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
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Immunocytochemistry/ Immunofluorescence - Anti-Nucleostemin antibody (ab70346)ICC/IF image of ab70346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70346, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Nucleostemin with ab70346 on formaldehyde-fixed paraffin-embedded human colon sections. Antigen retrieval step: heat mediated in citric acid pH6 buffer. Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody ab70346 incubated at 1/50 for 16 hours in TBS/BSA/azide. Secondary antibody: anti-rabbit IgG conjugated to biotin (1/200). The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. Stem cell markers have been observed in cycling columnar cells at the crypt base (Nature 449, 1003-1007; 2007). The cells (nuclei of the simple columnar epithelium of the upper mucosa) immunoreactive for Nucleostemin in this image (green arrowheads) anatomically correspond to the same cells expected to be Lgr5 positivite. A red arrowhead indicates nucleolar positivity in one of many cell nuclei in the Lamina propria of the mucosa (this area is not known for stem cell posit
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab70346 has been referenced in 11 publications.
- Sukocheva OA et al. Divergence of Intracellular Trafficking of Sphingosine Kinase 1 and Sphingosine-1-Phosphate Receptor 3 in MCF-7 Breast Cancer Cells and MCF-7-Derived Stem Cell-Enriched Mammospheres. Int J Mol Sci 22:N/A (2021). PubMed: 33919234
- Zhang X et al. Nucleostemin promotes hepatocellular carcinoma by regulating the function of STAT3. Exp Cell Res 387:111748 (2020). PubMed: 31785228
- Ho TC et al. PEDF-derived peptide promotes tendon regeneration through its mitogenic effect on tendon stem/progenitor cells. Stem Cell Res Ther 10:2 (2019). PubMed: 30606221
- Francipane MG et al. Establishment and Characterization of 5-Fluorouracil-Resistant Human Colorectal Cancer Stem-Like Cells: Tumor Dynamics under Selection Pressure. Int J Mol Sci 20:N/A (2019). PubMed: 31013771
- Yildirim GD et al. High nucleostemin expression has a favorable prognostic effect on gastric carcinomas Turk J Med Sci 48:484-490 (2018). PubMed: 29914242
