Recombinant
RabMAb

Recombinant Anti-Nucleostemin antibody [EPR3911(2)] (ab129185)

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Overview

  • Product name

    Anti-Nucleostemin antibody [EPR3911(2)]
    See all Nucleostemin primary antibodies

  • Description

    Rabbit monoclonal [EPR3911(2)] to Nucleostemin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human Nucleostemin (Q9BVP2).

  • Positive control

    • HeLa, PC-3, SW480, and K562 cell lysates, Permeabilized SW480 cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.

    • Tissue specificity

      Increased levels in lung tissue in cancer patients.

    • Sequence similarities

      Belongs to the MMR1/HSR1 GTP-binding protein family.
      Contains 1 G (guanine nucleotide-binding) domain.

    • Domain

      The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.
      In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

    • Cellular localization

      Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.
    • Information by UniProt

    • Database links

      • Alternative names

        • C77032 antibody
        • E2 induced gene 3 protein antibody
        • E2-induced gene 3 protein antibody
        • E2IG3 antibody
        • Estradiol induced nucleotide binding protein antibody
        • GNL3 antibody
        • GNL3_HUMAN antibody
        • Guanine nucleotide binding protein like 3 (nucleolar) antibody
        • Guanine nucleotide binding protein like 3 antibody
        • Guanine nucleotide-binding protein-like 3 antibody
        • MGC800 antibody
        • NNP47 antibody
        • Novel nucleolar protein 47 antibody
        • NS antibody
        • Nucleolar GTP binding protein 3 antibody
        • Nucleolar GTP-binding protein 3 antibody
        • Nucleostemin antibody
        • Putative nucleotide binding protein estradiol induced antibody
        see all

      Images

      • Western blot - Anti-Nucleostemin antibody [EPR3911(2)] (ab129185)
        Western blot - Anti-Nucleostemin antibody [EPR3911(2)] (ab129185)
        All lanes : Anti-Nucleostemin antibody [EPR3911(2)] (ab129185) at 1/1000 dilution

        Lane 1 : HeLa cell lysates
        Lane 2 : PC-3 cell lysates
        Lane 3 : SW480 cell lysates
        Lane 4 : K562 cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 62 kDa

      • Flow Cytometry - Anti-Nucleostemin antibody [EPR3911(2)] (ab129185)
        Flow Cytometry - Anti-Nucleostemin antibody [EPR3911(2)] (ab129185)
        Flow cytometric analysis of permeabilized SW480 cells using ab129185 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
      • Other - Anti-Nucleostemin antibody [EPR3911(2)] (ab129185)
        Other - Anti-Nucleostemin antibody [EPR3911(2)] (ab129185)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab129185 has not yet been referenced specifically in any publications.

      Publishing research using ab129185? Please let us know so that we can cite the reference in this datasheet.

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