Product nameAnti-NUDC antibody [EPR5073]
See all NUDC primary antibodies
DescriptionRabbit monoclonal [EPR5073] to NUDC
Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human NUDC aa 300-400. The exact sequence is proprietary.
- WB: HepG2, 293T, HeLa and SH-SY5Y cell lysates IHC-P: Human brain tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109318 in the following tested applications.
|WB||1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPlays a role in neurogenesis and neuronal migration (By similarity). Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.
Tissue specificityUbiquitous. Highly expressed in fetal liver, kidney, lung and brain. Highly expressed in adult pancreas, kidney, skeletal muscle, liver, lung, placenta, prostate, brain and heart.
Sequence similaritiesBelongs to the nudC family.
Contains 1 CS domain.
modificationsReversibly phosphorylated on serine residues during the M phase of the cell cycle. Phosphorylation on Ser-274 and Ser-326 is necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Phosphorylated by PLK and other kinases.
Cellular localizationCytoplasm > cytoskeleton. Nucleus. In a filamentous pattern adjacent to the nucleus of migrating cerebellar granule cells. Colocalizes with tubulin and dynein and with the microtubule organizing center. Distributed throughout the cytoplasm of non-migrating cells. A small proportion is nuclear, in a punctate pattern.
- Information by UniProt
- HNUDC antibody
- MNUDC antibody
- MNUDC protein antibody
All lanes : Anti-NUDC antibody [EPR5073] (ab109318) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : 293T lysate
Lane 3 : HeLa lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of NUDC in paraffin embedded Human brain tissue, using ab109318 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab109318 has not yet been referenced specifically in any publications.