Recombinant Anti-NUDCD2 antibody [EPR11558] (ab171979)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11558] to NUDCD2
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NUDCD2 antibody [EPR11558]
See all NUDCD2 primary antibodies -
Description
Rabbit monoclonal [EPR11558] to NUDCD2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, 293T and HepG2 cell lysates; Human colon and Human endometrial adenocarcinoma tissues; HepG2 and 293T cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11558 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/100 - 1/250.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 18 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/5000. Predicted molecular weight: 18 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Contains 1 CS domain. - Information by UniProt
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Database links
- Entrez Gene: 134492 Human
- Entrez Gene: 52653 Mouse
- Entrez Gene: 287199 Rat
- SwissProt: Q8WVJ2 Human
- SwissProt: Q9CQ48 Mouse
- SwissProt: Q5M823 Rat
- Unigene: 140443 Human
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Alternative names
- NudC domain containing 2 antibody
- NudC domain-containing protein 2 antibody
- NudC like protein 2 antibody
see all
Images
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All lanes : Anti-NUDCD2 antibody [EPR11558] (ab171979) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 18 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NUDCD2 with ab171979 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling NUDCD2 with ab171979 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized 293T cells labeling NUDCD2 with ab171979 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).
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Immunofluorescent analysis of HepG2 cells labeling NUDCD2 with ab171979 at 1/100 dilution.
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Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling NUDCD2 with ab171979 at 1/10 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab171979 has not yet been referenced specifically in any publications.