Recombinant
RabMAb

Anti-NUDCD2 antibody [EPR11558] (ab171979)

Overview

  • Product name
    Anti-NUDCD2 antibody [EPR11558]
    See all NUDCD2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11558] to NUDCD2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NUDCD2 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8WVJ2

  • Positive control
    • K562, 293T and HepG2 cell lysates; Human colon and Human endometrial adenocarcinoma tissues; HepG2 and 293T cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11558
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 18 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-NUDCD2 antibody [EPR11558] (ab171979) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 18 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NUDCD2 with ab171979 at 1/50 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling NUDCD2 with ab171979 at 1/50 dilution.

  • Flow cytometric analysis of permeabilized 293T cells labeling NUDCD2 with ab171979 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).

  • Immunofluorescent analysis of HepG2 cells labeling NUDCD2 with ab171979 at 1/100 dilution.

  • Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling NUDCD2 with ab171979 at 1/10 dilution.

References

ab171979 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab171979.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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