Overview

  • Product name

  • Description

    Rabbit polyclonal to NUDCD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human NUDCD3 aa 89-160.
    Sequence:

    RRKEEEEAKTVSAAAAEKEPVPVPVQEIEIDSTTELDGHQEVEKVQPPGP VKEMAHGSQEAEAPGAVAGAAE


    Database link: Q8IVD9

  • Positive control

    • IHC-P: Human rectum tissue. ICC/IF: U-251 MG cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

Target

  • Relevance

    NUDCD3 functions to maintain the stability of dynein intermediate chain. Depletion of NUDCD3 results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. NUDCD3 levels increase after the G1/S transition.
  • Database links

  • Alternative names

    • KIAA1068 antibody
    • NudC domain containing 3 antibody
    • NudC domain containing protein 3 antibody
    • NudC like protein antibody
    • NUDCD 3 antibody
    • NudCL antibody
    • OTTHUMP00000159457 antibody
    see all

Images

  • Paraffin-embedded human rectum tissue stained for NUDCD3 with ab224415 (1/500) in immunohistochemical analysis.

  • PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for NUDCD3 (green) using ab224415 (4 µg/ml) in ICC/IF.

References

ab224415 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab224415.
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