Overview

  • Product name

  • Description

    Rabbit polyclonal to NUDCD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human NUDCD3 aa 100-150. The exact sequence is proprietary.
    Database link: Q8IVD9

  • Positive control

    • IP: NUDCD3 IP in HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to NUDCD3 immobilized on solid support
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance

      NUDCD3 functions to maintain the stability of dynein intermediate chain. Depletion of NUDCD3 results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. NUDCD3 levels increase after the G1/S transition.
    • Database links

    • Alternative names

      • KIAA1068 antibody
      • NudC domain containing 3 antibody
      • NudC domain containing protein 3 antibody
      • NudC like protein antibody
      • NUDCD 3 antibody
      • NudCL antibody
      • OTTHUMP00000159457 antibody
      see all

    Images

    • NUDCD3 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
      ab241970 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.

      Lane 1: ab241970 IP in HEK-293T whole cell lysate.
      Lane 2: Control IgG.

      Chemiluminescence detection: 10 seconds.

    References

    ab241970 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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