Product nameAnti-NUDCD3 antibody
See all NUDCD3 primary antibodies
DescriptionRabbit polyclonal to NUDCD3
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human NUDCD3 aa 100-150. The exact sequence is proprietary.
Database link: Q8IVD9
- IP: NUDCD3 IP in HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to NUDCD3 immobilized on solid support
Our Abpromise guarantee covers the use of ab241970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceNUDCD3 functions to maintain the stability of dynein intermediate chain. Depletion of NUDCD3 results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. NUDCD3 levels increase after the G1/S transition.
- KIAA1068 antibody
- NudC domain containing 3 antibody
- NudC domain containing protein 3 antibody
NUDCD3 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241970 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.
Lane 1: ab241970 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 10 seconds.
ab241970 has not yet been referenced specifically in any publications.