Key features and details
- Rabbit polyclonal to NUDT12
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NUDT12 antibody
DescriptionRabbit polyclonal to NUDT12
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Orangutan
Recombinant fragment corresponding to Human NUDT12 aa 167-356. (BC041099).
ESFQ QPEVRLCQLN YTDIKDYLAQ PEKITLIFLG VELEIKDKLL NYAGEVPREE EDGLVAWFAL GIDPIAAEEF KQRHENCYFL HPPMPALLQL KEKEAGVVAQ ARSVLAWHSR YKFCPTCGNA TKIEEGGYKR LCLKEDCPSL NGVHNTSYPR VDPVVIMQVI HPDGTKCLLG RQKRFPPGMF TCLAGF
Database link: Q9BQG2
- HeLa, 293T, A549 and HepG2 cell lysates; Human fetal stomach tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Reconstitute in 200 ul sterile H2O.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/1000. Predicted molecular weight: 52 kDa.|
|IHC-P||1/100 - 1/500.|
RelevanceNucleotides are involved in numerous biochemical reactions and pathways within the cell as substrates, cofactors, and effectors. Nudix hydrolases, such as NUDT12 (Nudix (nucleoside diphosphate linked moiety X)-type motif 12), regulate the concentrations of individual nucleotides and of nucleotide ratios in response to changing circumstances. NUDT12 hydrolyzes NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. It also has moderate activity towards NAD(P)(+), ADP-ribose and diadenosine triphosphate. It may regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism.
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All lanes : Anti-NUDT12 antibody (ab197310) at 1/500 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Predicted band size: 52 kDa
Immunohistochemical analysis of formaliln-fixed, paraffin-embedded Human fetal stomach tissue labeling NUDT12 with ab197310 at 1/100 dilution.
ab197310 has been referenced in 1 publication.
- Grudzien-Nogalska E et al. Structural and mechanistic basis of mammalian Nudt12 RNA deNADding. Nat Chem Biol 15:575-582 (2019). PubMed: 31101919