Overview

  • Product name

    Anti-NUDT19 antibody [EPR13162-63]
    See all NUDT19 primary antibodies
  • Description

    Rabbit monoclonal [EPR13162-63] to NUDT19
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NUDT19. The exact sequence is proprietary.
    Database link: A8MXV4

  • Positive control

    • Human heart and kidney tissues. K562, 293T, Human ovary and Human spleen lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Coenzyme A diphosphatase that mediates the hydrolysis of a wide range of CoA esters, including choloyl-CoA and branched-chain fatty-acyl-CoA esters. At low substrate concentrations medium and long-chain fatty-acyl-CoA esters are the primary substrates.
    • Sequence similarities

      Belongs to the Nudix hydrolase family.
      Contains 1 nudix hydrolase domain.
    • Cellular localization

      Peroxisome. Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • mitochondrial antibody
      • Nucleoside diphosphate-linked moiety X motif 19 antibody
      • nucleoside diphosphate-linked moiety X motif 19, mitochondrial antibody
      • NUD19_HUMAN antibody
      • nudix (nucleoside diphosphate linked moiety X)-type motif 19 antibody
      • Nudix motif 19 antibody
      • NUDT19 antibody
      • RP2 antibody
      see all

    Images

    • All lanes : Anti-NUDT19 antibody [EPR13162-63] (ab180166) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Human ovary lysate
      Lane 4 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 42 kDa

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling NUDT19 using ab180166 at 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NUDT19 using ab180166 at 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunofluorescence analysis of 293T cells labeling NUDT19 using ab180166 at 1/100 (green). DAPI nuclear staining (blue).

    References

    ab180166 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab180166.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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