Overview

  • Product name
  • Description
    Rabbit polyclonal to NUDT21
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment within Human NUDT21 aa 1-227. The exact sequence is proprietary.
    Database link: O43809

  • Positive control
    • SK-N-SH , IMR32 and SK-N-AS whole cell lysate/extracts; mouse fore brain, mouse ovary and rat middle brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 79% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.

Target

  • Function
    Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.
  • Sequence similarities
    Belongs to the Nudix hydrolase family. CPSF5 subfamily.
    Contains 1 nudix hydrolase domain.
  • Post-translational
    modifications
    Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 AND SIRT2.
  • Cellular localization
    Nucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFIM 25 antibody
    • CFIM25 antibody
    • Cleavage and polyadenylation specific factor 5 25 kD subunit antibody
    • Cleavage and polyadenylation specific factor 5 25 kDa antibody
    • Cleavage and polyadenylation specific factor 5 antibody
    • Cleavage and polyadenylation specificity factor 25 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 5 antibody
    • Cleavage factor Im complex 25 kDa subunit antibody
    • CPSF 25 antibody
    • CPSF 25 kDa subunit antibody
    • CPSF 5 antibody
    • CPSF25 antibody
    • CPSF5 antibody
    • CPSF5_HUMAN antibody
    • DKFZp686H1588 antibody
    • Nucleoside diphosphate linked moiety X motif 21 antibody
    • Nucleoside diphosphate-linked moiety X motif 21 antibody
    • Nudix (nucleoside diphosphate linked moiety X) type motif 21 antibody
    • Nudix hydrolase 21 antibody
    • Nudix motif 21 antibody
    • NUDT 21 antibody
    • Nudt21 antibody
    • Pre mRNA cleavage factor Im (25kD) antibody
    • Pre mRNA cleavage factor Im 25 kDa subunit antibody
    • Pre mRNA cleavage factor Im 25kD subunit antibody
    • Pre-mRNA cleavage factor Im 25 kDa subunit antibody
    see all

Images

  • All lanes : Anti-NUDT21 antibody (ab183660) at 1/1000 dilution

    Lane 1 : SK-N-SH whole cell lysate/extract
    Lane 2 : IMR32 whole cell lysate/extract
    Lane 3 : SK-N-AS whole cell lysate/extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12 % SDS-PAGE

  • Immunohistochemical analysis of paraffin-embedded mouse fore brain tissue labeling NUDT21 with ab183660 at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue labeling NUDT21 with ab183660 at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded rat middle brain tissue labeling NUDT21 with ab183660 at 1/500 dilution.

References

This product has been referenced in:
  • Wang Y  et al. CFIm25 inhibits hepatocellular carcinoma metastasis by suppressing the p38 and JNK/c-Jun signaling pathways. Oncotarget 9:11783-11793 (2018). IHC-P ; Human . Read more (PubMed: 29545935) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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