Overview

  • Product name
  • Description
    Rabbit polyclonal to NUDT21
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: INLYPLTNYT FGTKEPLYEK DSSVAARFQR MREEFDKIGM RRTVEGVLIV, corresponding to internal sequence amino acids 37-86 of Human NUDT21

  • Positive control
    • HepG2 cell lysate Human intestine tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • Function
    Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.
  • Sequence similarities
    Belongs to the Nudix hydrolase family. CPSF5 subfamily.
    Contains 1 nudix hydrolase domain.
  • Post-translational
    modifications
    Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 AND SIRT2.
  • Cellular localization
    Nucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFIM 25 antibody
    • CFIM25 antibody
    • Cleavage and polyadenylation specific factor 5 25 kD subunit antibody
    • Cleavage and polyadenylation specific factor 5 25 kDa antibody
    • Cleavage and polyadenylation specific factor 5 antibody
    • Cleavage and polyadenylation specificity factor 25 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 5 antibody
    • Cleavage factor Im complex 25 kDa subunit antibody
    • CPSF 25 antibody
    • CPSF 25 kDa subunit antibody
    • CPSF 5 antibody
    • CPSF25 antibody
    • CPSF5 antibody
    • CPSF5_HUMAN antibody
    • DKFZp686H1588 antibody
    • Nucleoside diphosphate linked moiety X motif 21 antibody
    • Nucleoside diphosphate-linked moiety X motif 21 antibody
    • Nudix (nucleoside diphosphate linked moiety X) type motif 21 antibody
    • Nudix hydrolase 21 antibody
    • Nudix motif 21 antibody
    • NUDT 21 antibody
    • Nudt21 antibody
    • Pre mRNA cleavage factor Im (25kD) antibody
    • Pre mRNA cleavage factor Im 25 kDa subunit antibody
    • Pre mRNA cleavage factor Im 25kD subunit antibody
    • Pre-mRNA cleavage factor Im 25 kDa subunit antibody
    see all

Images

  • All lanes : Anti-NUDT21 antibody (ab66667) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling NUDT21 with ab6667 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnification: 400X.

References

ab66667 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

According to our records, ab56856 was proving difficult to use in WB and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand t...

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I have read the details, you have kindly provided and have few suggestions; these might improve the results.

- NUDT21 as per htt...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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