Product nameAnti-NUDT21/CFIM25 antibody
See all NUDT21/CFIM25 primary antibodies
DescriptionRabbit polyclonal to NUDT21/CFIM25
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
- HepG2 cell lysate Human intestine tissue
This product was previously labelled as NUDT21
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab66667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
FunctionComponent of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.
Sequence similaritiesBelongs to the Nudix hydrolase family. CPSF5 subfamily.
Contains 1 nudix hydrolase domain.
modificationsAcetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 AND SIRT2.
Cellular localizationNucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
- Information by UniProt
- CFIM 25 antibody
- CFIM25 antibody
- Cleavage and polyadenylation specific factor 5 25 kD subunit antibody
All lanes : Anti-NUDT21/CFIM25 antibody (ab66667) at 1.25 µg/ml
Lane 1 : Marker
Lane 2 : HepG2 cell lysate at 10 µg
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling NUDT21/CFIM25 with ab6667 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnification: 400X.
ab66667 has not yet been referenced specifically in any publications.