Product nameAnti-NUDT22 antibody
DescriptionRabbit polyclonal to NUDT22
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- WB: NUDT22 overexpression HEK-293T lysate. IHC-P: Human pancreas tissue. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.4 µg/ml. Predicted molecular weight: 33 kDa.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
Sequence similaritiesBelongs to the Nudix hydrolase family.
Contains 1 nudix hydrolase domain.
DomainAlthough related to the NUDIX hydrolase family, it lacks the Nudix box and does not seem to act as a hydrolase.
- Information by UniProt
- MGC13045 antibody
- Nucleoside diphosphate-linked moiety X motif 22 antibody
- NUD22_HUMAN antibody
PFA fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells labeling NUDT22 using ab243577 at 4 µg/ml (green) in ICC/IF. Microtubules in red.
All lanes : Anti-NUDT22 antibody (ab243577) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : NUDT22 overexpression HEK-293T lysate
Predicted band size: 33 kDa
Formalin-fixed, paraffin-embedded human pancreas tissue stained for NUDT22 with ab243577 at 1/20 dilution in immunohistochemical analysis.
ab243577 has not yet been referenced specifically in any publications.