Recombinant Anti-NUDT5 antibody [EPR7734] (ab129172)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7734] to NUDT5
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NUDT5 antibody [EPR7734]
See all NUDT5 primary antibodies -
Description
Rabbit monoclonal [EPR7734] to NUDT5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human NUDT5 aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: U937, HeLa, 293T and HepG2 cell lysates. IHC-P: Human kidney and Human bladder carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.77 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7734 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Hydrolyzes with similar activities ADP-ribose, ADP-mannose, and ADP-glucose. Can also hydrolyze other nucleotide sugars with low activity. -
Tissue specificity
Widely expressed. Most abundant in liver. -
Sequence similarities
Belongs to the Nudix hydrolase family.
Contains 1 nudix hydrolase domain. - Information by UniProt
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Database links
- Entrez Gene: 11164 Human
- Entrez Gene: 53893 Mouse
- Entrez Gene: 361274 Rat
- Omim: 609230 Human
- SwissProt: Q9UKK9 Human
- SwissProt: Q9JKX6 Mouse
- SwissProt: Q6AY63 Rat
- Unigene: 555956 Human
see all -
Alternative names
- ADP sugar pyrophosphatase antibody
- ADP-sugar pyrophosphatase antibody
- hYSAH 1 antibody
see all
Images
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All lanes : Anti-NUDT5 antibody [EPR7734] (ab129172) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : NUDT5 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : U-937 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab129172 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.
ab129172 Anti-NUDT5 antibody [EPR7734] was shown to specifically react with NUDT5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266242 (knockout cell lysate ab258087) was used. Wild-type and NUDT5 knockout samples were subjected to SDS-PAGE. ab129172 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab129172, at a 1/50, staining NUDT5 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-NUDT5 antibody [EPR7734] (ab129172) at 1/1000 dilution
Lane 1 : U937 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 24 kDa -
ab129172, at 1/50, staining NUDT5 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab129172 has been referenced in 5 publications.
- Qi H et al. The ADP-ribose hydrolase NUDT5 is important for DNA repair. Cell Rep 41:111866 (2022). PubMed: 36543120
- Zhang H et al. The high expression of NUDT5 indicates poor prognosis of breast cancer by modulating AKT / Cyclin D signaling. PLoS One 16:e0245876 (2021). PubMed: 33571243
- Wang JJ et al. The high expression of MTH1 and NUDT5 predict a poor survival and are associated with malignancy of esophageal squamous cell carcinoma. PeerJ 8:e9195 (2020). PubMed: 32518727
- Lin YT et al. Hepatitis B Virus X Protein Increases 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine (8-Oxodg) Level via Repressing MTH1/ MTH2 Expression in Hepatocytes. Cell Physiol Biochem 51:80-96 (2018). PubMed: 30448843
- Li J et al. MutT-related proteins are novel progression and prognostic markers for colorectal cancer. Oncotarget 8:105714-105726 (2017). PubMed: 29285286