Recombinant
RabMAb

Recombinant Anti-NUDT5 antibody [EPR7735] (ab129163)

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Overview

  • Product name

    Anti-NUDT5 antibody [EPR7735]
    See all NUDT5 primary antibodies

  • Description

    Rabbit monoclonal [EPR7735] to NUDT5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human NUDT5 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, HepG2 and U937 cell lysates

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 24 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Hydrolyzes with similar activities ADP-ribose, ADP-mannose, and ADP-glucose. Can also hydrolyze other nucleotide sugars with low activity.

    • Tissue specificity

      Widely expressed. Most abundant in liver.

    • Sequence similarities

      Belongs to the Nudix hydrolase family.
      Contains 1 nudix hydrolase domain.
    • Information by UniProt

    • Database links

      • Alternative names

        • ADP sugar pyrophosphatase antibody
        • ADP-sugar pyrophosphatase antibody
        • hYSAH 1 antibody
        • hYSAH1 antibody
        • Nucleoside diphosphate linked moiety X motif 5 antibody
        • Nucleoside diphosphate linked moiety X type motif 5 antibody
        • Nucleoside diphosphate-linked moiety X motif 5 antibody
        • Nudix (nucleoside diphosphate linked moiety X) type motif 5 antibody
        • Nudix motif 5 antibody
        • Nudix type motif 5 antibody
        • NUDT 5 antibody
        • Nudt5 antibody
        • NUDT5_HUMAN antibody
        • YSA1 antibody
        • YSA1H antibody
        see all

      Images

      • Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)
        Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)
        All lanes : Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : 293T cell lysate
        Lane 3 : HepG2 cell lysate
        Lane 4 : U937 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

        Predicted band size: 24 kDa
        Observed band size: 33 kDa
        why is the actual band size different from the predicted?

      • Other - Anti-NUDT5 antibody [EPR7735] (ab129163)
        Other - Anti-NUDT5 antibody [EPR7735] (ab129163)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab129163 has not yet been referenced specifically in any publications.

      Publishing research using ab129163? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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