Recombinant Anti-NUDT5 antibody [EPR7735] (ab129163)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-NUDT5 antibody [EPR7735]
See all NUDT5 primary antibodies -
Description
Rabbit monoclonal [EPR7735] to NUDT5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NUDT5 aa 150-250. The exact sequence is proprietary.
-
Positive control
- HeLa, 293T, HepG2 and U937 cell lysates
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.48 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7735 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab129163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 24 kDa). | |
| IP | 1/10 - 1/100. |
Target
-
Function
Hydrolyzes with similar activities ADP-ribose, ADP-mannose, and ADP-glucose. Can also hydrolyze other nucleotide sugars with low activity. -
Tissue specificity
Widely expressed. Most abundant in liver. -
Sequence similarities
Belongs to the Nudix hydrolase family.
Contains 1 nudix hydrolase domain. - Information by UniProt
-
Database links
- Entrez Gene: 11164 Human
- Omim: 609230 Human
- SwissProt: Q9UKK9 Human
- Unigene: 555956 Human
-
Alternative names
- ADP sugar pyrophosphatase antibody
- ADP-sugar pyrophosphatase antibody
- hYSAH 1 antibody
see all
Images
-
![Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)](https://www.abcam.com/ps/products/129/ab129163/Images/ab129163-143163-anti-nudt5-antibody-epr7735-western-blot.jpg)
Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)All lanes : Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted? -
![Other - Anti-NUDT5 antibody [EPR7735] (ab129163)](https://www.abcam.com/ps/products/129/ab129163/Images/ab129163-143162-anti-nudt5-antibody-epr7735.jpg)
Other - Anti-NUDT5 antibody [EPR7735] (ab129163)
Datasheets and documents
References
ab129163 has not yet been referenced specifically in any publications.
