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  1. Link

    nudt5-antibody-epr7735-ab129163.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA / Nucleotides
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-NUDT5 antibody [EPR7735] (ab129163)

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Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)
  • Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)
  • OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] (ab129163)
  • Anti-NUDT5 antibody [EPR7735] (ab129163)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7735] to NUDT5
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Knockout
Product image
Human NUDT5 knockout HEK-293T cell line (ab266242)
Primary
Product image
Anti-RBM25 antibody (ab72237)

View more associated products

Overview

  • Product name

    Anti-NUDT5 antibody [EPR7735]
    See all NUDT5 primary antibodies
  • Description

    Rabbit monoclonal [EPR7735] to NUDT5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NUDT5 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, 293T, HepG2 and U937 cell lysates
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 3.48 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7735
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (ab248321)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human NUDT5 knockout HEK-293T cell line (ab266242)
  • KO cell lysates

    • Human NUDT5 knockout HEK-293T cell lysate (ab258087)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 24 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 24 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Function

    Hydrolyzes with similar activities ADP-ribose, ADP-mannose, and ADP-glucose. Can also hydrolyze other nucleotide sugars with low activity.
  • Tissue specificity

    Widely expressed. Most abundant in liver.
  • Sequence similarities

    Belongs to the Nudix hydrolase family.
    Contains 1 nudix hydrolase domain.
  • Target information above from: UniProt accession Q9UKK9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 11164 Human
    • Omim: 609230 Human
    • SwissProt: Q9UKK9 Human
    • Unigene: 555956 Human
    • Alternative names

      • ADP sugar pyrophosphatase antibody
      • ADP-sugar pyrophosphatase antibody
      • hYSAH 1 antibody
      • hYSAH1 antibody
      • Nucleoside diphosphate linked moiety X motif 5 antibody
      • Nucleoside diphosphate linked moiety X type motif 5 antibody
      • Nucleoside diphosphate-linked moiety X motif 5 antibody
      • Nudix (nucleoside diphosphate linked moiety X) type motif 5 antibody
      • Nudix motif 5 antibody
      • Nudix type motif 5 antibody
      • NUDT 5 antibody
      • Nudt5 antibody
      • NUDT5_HUMAN antibody
      • YSA1 antibody
      • YSA1H antibody
      see all

    Images

    • Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)
      Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)
      All lanes : Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : NUDT5 knockout HEK293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 24 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab129163 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab129163 Anti-NUDT5 antibody [EPR7735] was shown to specifically react with NUDT5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266242 (knockout cell lysate ab258087) was used. Wild-type and NUDT5 knockout samples were subjected to SDS-PAGE. ab129163 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)
      Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)
      All lanes : Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : U937 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 24 kDa
      Observed band size: 33 kDa why is the actual band size different from the predicted?

    • OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] (ab129163)
      OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] (ab129163)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-NUDT5 antibody [EPR7735] (ab129163)
      Anti-NUDT5 antibody [EPR7735] (ab129163)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab129163? Please let us know so that we can cite the reference in this datasheet.

    ab129163 has not yet been referenced specifically in any publications.

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