Key features and details
- Rabbit polyclonal to NUDT8
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NUDT8 antibody
DescriptionRabbit polyclonal to NUDT8
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human colon tissue. WB: RT4 cell lysate. ICC/IF: U-251 MG cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 25 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionProbably mediates the hydrolysis of some nucleoside diphosphate derivatives.
Sequence similaritiesBelongs to the Nudix hydrolase family.
Contains 1 nudix hydrolase domain.
- Information by UniProt
- FLJ41567 antibody
- Nucleoside diphosphate linked moiety X motif 8 antibody
- Nucleoside diphosphate linked moiety X motif 8 mitochondrial antibody
PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling NUDT8 using ab243729 at 4 µg/ml (green) in ICC/IF.
Anti-NUDT8 antibody (ab243729) at 0.4 µg/ml + RT4 (Human urinary bladder cancer cell line) cell lysate
Predicted band size: 25 kDa
Formalin-fixed, paraffin-embedded human colon tissue stained for NUDT8 with ab243729 at a 1/20 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243729 has not yet been referenced specifically in any publications.