Anti-NUDT8 antibody (ab243729)

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Overview

  • Product name

    Anti-NUDT8 antibody

  • Description

    Rabbit polyclonal to NUDT8

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human NUDT8 aa 138-236.
    Sequence:

    EVFALPLAHLLQTQNQGYTHFCRGGHFRYTLPVFLHGPHRVWGLTAVITE FALQLLAPGTYQPRLAGLTCSGAEGLARPKQPLASPCQASSTPGLNKGL


    Database link: Q8WV74

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human colon tissue. WB: RT4 cell lysate. ICC/IF: U-251 MG cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab243729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.4 µg/ml. Predicted molecular weight: 25 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function

    Probably mediates the hydrolysis of some nucleoside diphosphate derivatives.

  • Sequence similarities

    Belongs to the Nudix hydrolase family.
    Contains 1 nudix hydrolase domain.

  • Cellular localization

    Mitochondrion.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ41567 antibody
      • Nucleoside diphosphate linked moiety X motif 8 antibody
      • Nucleoside diphosphate linked moiety X motif 8 mitochondrial antibody
      • Nucleoside diphosphate-linked moiety X motif 8, mitochondrial antibody
      • Nudix (nucleoside diphosphate linked moiety X) type motif 8 antibody
      • Nudix motif 8 antibody
      • Nudix type motif 8 antibody
      • NUDT 8 antibody
      • NUDT8 antibody
      • NUDT8_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-NUDT8 antibody (ab243729)
      Immunocytochemistry/ Immunofluorescence - Anti-NUDT8 antibody (ab243729)

      PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling NUDT8 using ab243729 at 4 µg/ml (green) in ICC/IF.

    • Western blot - Anti-NUDT8 antibody (ab243729)
      Western blot - Anti-NUDT8 antibody (ab243729)
      Anti-NUDT8 antibody (ab243729) at 0.4 µg/ml + RT4 (Human urinary bladder cancer cell line) cell lysate

      Predicted band size: 25 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT8 antibody (ab243729)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT8 antibody (ab243729)

      Formalin-fixed, paraffin-embedded human colon tissue stained for NUDT8 with ab243729 at a 1/20 dilution in immunohistochemical analysis.

    References

    ab243729 has not yet been referenced specifically in any publications.

    Publishing research using ab243729? Please let us know so that we can cite the reference in this datasheet.

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