Overview

  • Product name

    Anti-NUDT9 antibody [EPR15175]
    See all NUDT9 primary antibodies
  • Description

    Rabbit monoclonal [EPR15175] to NUDT9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NUDT9 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BW91

  • Positive control

    • WB: Jurkat and HUVEC cell lysates. IHC-P: Human and rat kidney tissues. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab197021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ICC/IF 1/250.
Flow Cyt 1/170.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Hydrolyzes ADP-ribose (ADPR) to AMP and ribose 5'-phosphate.
  • Tissue specificity

    Ubiquitously expressed but isoform 1 is the most predominant isoform.
  • Sequence similarities

    Belongs to the Nudix hydrolase family. NudF subfamily.
    Contains 1 nudix hydrolase domain.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenosine diphosphoribose pyrophosphatase antibody
    • ADP ribose diphosphatase antibody
    • ADP ribose phosphohydrolase antibody
    • ADP ribose pyrophosphatase, mitochondrial precursor antibody
    • ADP ribose pyrosphosphatase NUDT9 antibody
    • ADP-ribose diphosphatase antibody
    • ADP-ribose phosphohydrolase antibody
    • ADP-ribose pyrophosphatase, mitochondrial antibody
    • ADPR PPase antibody
    • ADPR-PPase antibody
    • EC 3.6.1.13 antibody
    • Nucleoside diphosphate linked moiety X motif 9 antibody
    • Nucleoside diphosphate linked moiety X type motif 9 antibody
    • Nucleoside diphosphate-linked moiety X motif 9 antibody
    • Nudix (nucleoside diphosphate linked moiety X) type motif 9 antibody
    • Nudix hydrolase 9 antibody
    • Nudix motif 9 antibody
    • NUDT10 antibody
    • NUDT9 antibody
    • NUDT9_HUMAN antibody
    see all

Images

  • All lanes : Anti-NUDT9 antibody [EPR15175] (ab197021) at 1/20000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate
    Lane 2 : HUVEC (Human umbilical vein endothelial cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NUDT9 with ab197021 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling NUDT9 with ab197021 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Rat kidney tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NUDT9 with ab197021 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).  Mitochondrion staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). COX IV is detected with ab33985 (anti-COX IV mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab197021 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab33985 (anti-COX IV mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NUDT9 with ab197021 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

ab197021 has not yet been referenced specifically in any publications.

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