RecombinantRabMAb

Recombinant Anti-NUDT9 antibody [EPR15175] (ab197021)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15175] to NUDT9
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-NUDT9 antibody [EPR15175]
    See all NUDT9 primary antibodies

  • Description

    Rabbit monoclonal [EPR15175] to NUDT9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NUDT9 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BW91

  • Positive control

    • WB: Jurkat and HUVEC cell lysates. IHC-P: Human and rat kidney tissues. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab197021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ICC/IF 1/250.
Flow Cyt 1/170.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Hydrolyzes ADP-ribose (ADPR) to AMP and ribose 5'-phosphate.

  • Tissue specificity

    Ubiquitously expressed but isoform 1 is the most predominant isoform.

  • Sequence similarities

    Belongs to the Nudix hydrolase family. NudF subfamily.
    Contains 1 nudix hydrolase domain.

  • Cellular localization

    Mitochondrion.
  • Information by UniProt

  • Database links

    • Alternative names

      • Adenosine diphosphoribose pyrophosphatase antibody
      • ADP ribose diphosphatase antibody
      • ADP ribose phosphohydrolase antibody
      • ADP ribose pyrophosphatase, mitochondrial precursor antibody
      • ADP ribose pyrosphosphatase NUDT9 antibody
      • ADP-ribose diphosphatase antibody
      • ADP-ribose phosphohydrolase antibody
      • ADP-ribose pyrophosphatase, mitochondrial antibody
      • ADPR PPase antibody
      • ADPR-PPase antibody
      • EC 3.6.1.13 antibody
      • Nucleoside diphosphate linked moiety X motif 9 antibody
      • Nucleoside diphosphate linked moiety X type motif 9 antibody
      • Nucleoside diphosphate-linked moiety X motif 9 antibody
      • Nudix (nucleoside diphosphate linked moiety X) type motif 9 antibody
      • Nudix hydrolase 9 antibody
      • Nudix motif 9 antibody
      • NUDT10 antibody
      • NUDT9 antibody
      • NUDT9_HUMAN antibody
      see all

    Images

    • Western blot - Anti-NUDT9 antibody [EPR15175] (ab197021)
      Western blot - Anti-NUDT9 antibody [EPR15175] (ab197021)
      All lanes : Anti-NUDT9 antibody [EPR15175] (ab197021) at 1/20000 dilution

      Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate
      Lane 2 : HUVEC (Human umbilical vein endothelial cell line) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 39 kDa
      Observed band size: 39 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT9 antibody [EPR15175] (ab197021)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT9 antibody [EPR15175] (ab197021)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NUDT9 with ab197021 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT9 antibody [EPR15175] (ab197021)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT9 antibody [EPR15175] (ab197021)

      Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling NUDT9 with ab197021 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Rat kidney tissue is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-NUDT9 antibody [EPR15175] (ab197021)
      Immunocytochemistry/ Immunofluorescence - Anti-NUDT9 antibody [EPR15175] (ab197021)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NUDT9 with ab197021 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).  Mitochondrion staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). COX IV is detected with ab33985 (anti-COX IV mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:-
      -ve control 1: ab197021 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab33985 (anti-COX IV mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Flow Cytometry - Anti-NUDT9 antibody [EPR15175] (ab197021)
      Flow Cytometry - Anti-NUDT9 antibody [EPR15175] (ab197021)

      Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NUDT9 with ab197021 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    • Anti-NUDT9 antibody [EPR15175] (ab197021)
      Anti-NUDT9 antibody [EPR15175] (ab197021)

    References (0)

    Publishing research using ab197021? Please let us know so that we can cite the reference in this datasheet.

    ab197021 has not yet been referenced specifically in any publications.

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