Overview

  • Product name

  • Description

    Rabbit polyclonal to Nuf2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Chicken, Human
    Predicted to work with: Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide:

    CGGDSYAKIDEKTAELKRKMFKMS

    , corresponding to C terminal amino acids 443-463 of Human Nuf2.

  • Positive control

    • HeLa cells and cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
ICC/IF 1/200 - 1/1000. Methanol fixation is recommended for immunofluorescence staining.

Target

  • Function

    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
  • Sequence similarities

    Belongs to the NUF2 family.
  • Post-translational
    modifications

    Can be phosphorylated by AURKA and AURKB.
  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancer/testis antigen 106 antibody
    • CDCA 1 antibody
    • CDCA1 antibody
    • Cell division cycle associated 1 antibody
    • Cell division cycle associated protein 1 antibody
    • Cell division cycle-associated protein 1 antibody
    • CT106 antibody
    • hNuf 2 antibody
    • hNuf2 antibody
    • hNuf2R antibody
    • hsNuf2 antibody
    • Kinetochore protein Nuf2 antibody
    • Nuf 2 antibody
    • NUF2 antibody
    • NUF2 NDC80 kinetochore complex component antibody
    • NUF2 NDC80 kinetochore complex component homolog antibody
    • NUF2, S. cerevisiae, homolog of antibody
    • NUF2_HUMAN antibody
    • NUF2R antibody
    see all

Images

  • Anti-Nuf2 antibody (ab122962) at 1/5000 dilution + HeLa cell lysate

    Predicted band size: 54 kDa

  • ab122962 at 1/500 dilution staining Nuf2 in Methanol-fixed HeLa cells by Immunofluorescence (green) showing localization to kinetochores at the stages of prometaphase to anaphase. Chromosomes were stained by DAPI (violet).

References

This product has been referenced in:

  • Petsalaki E  et al. Chmp4c is required for stable kinetochore-microtubule attachments. Chromosoma N/A:N/A (2018). Read more (PubMed: 29968190) »
  • Hu P  et al. siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo. Biosci Rep 35:N/A (2015). WB, IHC-P ; Human . Read more (PubMed: 25370920) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Colon carcinoma)
Permeabilization
No
Specification
Colon carcinoma
Fixative
Methanol

Dr. Eleni Petsalaki

Verified customer

Submitted Feb 01 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up