Key features and details
- Rabbit polyclonal to Nuf2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Nuf2 antibody
See all Nuf2 primary antibodies
DescriptionRabbit polyclonal to Nuf2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human Nuf2 aa 165-464.
KLERLDSVPVEEQEEFKQLSDGIQELQQSLNQDFHQKTIVLQEGNSQKKS NISEKTKRLNELKLSVVSLKEIQESLKTKIVDSPEKLKNYKEKMKDTVQK LKNARQEVVEKYEIYGDSVDCLPSCQLEVQLYQKKIQDLSDNREKLASIL KESLNLEDQIESDESELKKLKTEENSFKRLMIVKKEKLATAQFKINKKHE DVKQYKRTVIEDCNKVQEKRGAVYERVTTINQEIQKIKLGIQQLKDAAER EKLKSQEIFLNLKTALEKYHDGIEKAAEDSYAKIDEKTAELKRKMFKMST
Database link: Q9BZD4
- WB: Mouse liver lysate; Jurkat, A431, PC-3, HEK-293T, HeLa and HepG2 whole cell lysate (ab7900). IHC-P: Human liver and skeletal muscle tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- Mouse liver tissue lysate - total protein (ab29301)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- Jurkat whole cell lysate (ab7899)
- A431 whole cell lysate (ab7909)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab230313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 54 kDa.|
FunctionActs as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Sequence similaritiesBelongs to the NUF2 family.
modificationsCan be phosphorylated by AURKA and AURKB.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
- Information by UniProt
- Cancer/testis antigen 106 antibody
- CDCA 1 antibody
- CDCA1 antibody
All lanes : Anti-Nuf2 antibody (ab230313) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 5 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 7 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 54 kDa
Paraffin-embedded human liver tissue stained for Nuf2 using ab230313 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for Nuf2 using ab230313 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230313 has not yet been referenced specifically in any publications.