Recombinant
RabMAb

Anti-Nuf2 antibody [EPR13018(B)] (ab176556)

Overview

  • Product name
    Anti-Nuf2 antibody [EPR13018(B)]
    See all Nuf2 primary antibodies
  • Description
    Rabbit monoclonal [EPR13018(B)] to Nuf2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Nuf2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BZD4

  • Positive control
    • 293T, A431, HeLa and Human fetal liver lysates; 293T cells.HEK 293 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR13018(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/500 - 1/1000.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
    • Sequence similarities
      Belongs to the NUF2 family.
    • Post-translational
      modifications
      Can be phosphorylated by AURKA and AURKB.
    • Cellular localization
      Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cancer/testis antigen 106 antibody
      • CDCA 1 antibody
      • CDCA1 antibody
      • Cell division cycle associated 1 antibody
      • Cell division cycle associated protein 1 antibody
      • Cell division cycle-associated protein 1 antibody
      • CT106 antibody
      • hNuf 2 antibody
      • hNuf2 antibody
      • hNuf2R antibody
      • hsNuf2 antibody
      • Kinetochore protein Nuf2 antibody
      • Nuf 2 antibody
      • NUF2 antibody
      • NUF2 NDC80 kinetochore complex component antibody
      • NUF2 NDC80 kinetochore complex component homolog antibody
      • NUF2, S. cerevisiae, homolog of antibody
      • NUF2_HUMAN antibody
      • NUF2R antibody
      see all

    Images

    • ab176556 staining Nuf2 in the human cell line 293T (Human epithelial cell line from embryonic kidney) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/60. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • ab176556 staining Nuf2 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a tubulin counterstain at a dilution of 1/200 and DAPI was used as a nuclear counterstain.

    • All lanes : Anti-Nuf2 antibody [EPR13018(B)] (ab176556) at 1/2000 dilution

      Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
      Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 54 kDa
      Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-Nuf2 antibody [EPR13018(B)] (ab176556) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 54 kDa

    • ab176556 staining Nuf2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

      See Abreview

    • Flow cytometry analysis of permeabilized 293T cells labeling Nuf2 (red), using ab176556 at a 1/10 dilution, or negative control rabbit IgG (green)

    References

    ab176556 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 05 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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