Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13019] to Nuf2
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-Nuf2 antibody [EPR13019]
See all Nuf2 primary antibodies
DescriptionRabbit monoclonal [EPR13019] to Nuf2
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Nuf2 aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: Q9BZD4
- HepG2 whole cell lysate (ab7900) can be used as a positive control in WB. HeLa, A431 and 293 cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab180945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 54 kDa.|
FunctionActs as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Sequence similaritiesBelongs to the NUF2 family.
modificationsCan be phosphorylated by AURKA and AURKB.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
- Information by UniProt
- Cancer/testis antigen 106 antibody
- CDCA 1 antibody
- CDCA1 antibody
All lanes : Anti-Nuf2 antibody [EPR13019] (ab180945) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
Lysate from 293 cells was immunoprecipitated with ab180945 at a 1/50 dilution. For the subsequent blot, ab180945 used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. Second lane is the negative control.
ab180945 has been referenced in 1 publication.
- Milev MP et al. TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment. J Cell Biol 209:221-34 (2015). WB, ICC/IF ; Human . PubMed: 25918224