Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13019] to Nuf2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Product nameAnti-Nuf2 antibody [EPR13019] - BSA and Azide free
See all Nuf2 primary antibodies
DescriptionRabbit monoclonal [EPR13019] to Nuf2 - BSA and Azide free
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human Nuf2 aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: Q9BZD4
Ab250277 is the carrier-free version of ab180945. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250277 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab250277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.|
FunctionActs as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Sequence similaritiesBelongs to the NUF2 family.
modificationsCan be phosphorylated by AURKA and AURKB.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
- Information by UniProt
- Cancer/testis antigen 106 antibody
- CDCA 1 antibody
- CDCA1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab250277 has not yet been referenced specifically in any publications.