Overview

  • Product name
    Anti-NuMA antibody [A73-B/D12]
    See all NuMA primary antibodies
  • Description
    Mouse monoclonal [A73-B/D12] to NuMA
  • Host species
    Mouse
  • Specificity
    Reacts with an intracellular 228 kD protein found in the nucleus during interphase.
  • Tested applications
    Suitable for: IP, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Live Ls 174T cells (human colon carcinoma).

  • Positive control
    • Tonsil, Thymus, Spleen.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    A73-B/D12
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-Fr 1/50 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/100 - 1/300. Detects a band of approximately 240 kDa.

Target

  • Function
    May be a structural component of the nucleus.
  • Cellular localization
    Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • Database links
    • Alternative names
      • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
      • NMP 22 antibody
      • Nuclear matrix protein 22 antibody
      • Nuclear mitotic apparatus protein 1 antibody
      • Nuclear mitotic apparatus protein antibody
      • NUMA 1 antibody
      • NUMA antibody
      • NuMA protein antibody
      • NUMA1 antibody
      • NUMA1_HUMAN antibody
      • SP H antigen antibody
      • SP-H antigen antibody
      • Structural nuclear protein antibody
      see all

    References

    This product has been referenced in:
    • Burch A  et al. A novel synaptic junction preparation for the identification and characterization of cleft proteins. PLoS One 12:e0174895 (2017). Read more (PubMed: 28362857) »
    • Han K  et al. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. Nature 503:72-7 (2013). Read more (PubMed: 24153177) »

    See all 4 Publications for this product

    Publishing research using ab5675? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    7 reviews or Q&A

    10
    Votes: Highest First

    In order to choose the best NuMA antibody for this application, I need to know the epitope-recognition sites. For Ab86129, you only indicate a range (a.a. 900 – 1000) and for Ab5675, I could not find this information. Can you disclose the epitope...

    Read More

    Thanks for your enquiry.

    Regarding the immunogen information:
    ab5675 immunogen is Live Ls 174T cells and epitope is not known.

    RE: could you provide Ab86129 and/or Ab84680 without BSA?
    These 2 antibodies are not typically ...

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NuMA antibody [A73-B/D12]

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Ovarian carcinoma)
    Specification
    Ovarian carcinoma
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Read More
    abreview image
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Mar 16 2009

    Thank you for your reply. I'd like to try a new vial, if it's possible. And about permeability, I am particulary interested in looking at colocalization of NuMA with our target protein, Mx during metaphase. During metaphase NuMA is supposed to localize...

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    Thank you for your e-mail. This is to let you know that we have placed a new order for you one vial of ab5675 as a replacement vial from a new batch. For your information, the new order number is 80286. We hope this new vial will work. Please do l...

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    I do not think your advice is helpful. First of all, using higher concentration of NuMA antibody is not good idea because I already observed very strong background when I usd 1:100 dilution of NuMA. Sounds like if this NuMA antibody can not even recogn...

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    Thank you for your reply. We are very sorry that you are not satisfied with our response. This antibody has been tested and characterized for IHC using human colon carcinoma. Unfortunately, we have no information if this product reacts with HeLa ce...

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    BATCH NUMBER 92289 DESCRIPTION OF THE PROBLEM Non-specific staining, I did not see any NuMA staining pattern I was supposed to see. For example, NuMA was supposed to be in the nucleus curing interphase, which I did not see. SAMPLE Sheep/...

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    Thank you for your enquiry. We are very sorry to hear that you are having problem with this antibody. We have looked at our computer system which stores all the technical enquiries, complaints and correspondence. Unfortunately, we have not received...

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    Would you have pictures of Immunofluorescence using this antibody on human cells ?

    Thank you for your enquiry. We unfortunately do not have an image available for ab5675. The antibody has been tested for application in Western blot, Immunohistochemistry (Formalin-fixed paraffin-embedded sections), and Immunohistochemistry (Frozen sec...

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    We received item ab5675 and noticed the color was red.... A few weeks ago a customer received item ab3076 that was also red. You sent me an e-mail stating item ab3076 should not be red. My question is item ab5675 suppose to be red??? Also we are still...

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    Thank you so much for your patience. I had to contact the originator of ab5675 and have just heard back. Concerning ab5675, the antibody is red in color because it is concentrated medium from cell culture containing phenol red. The other antibody, ab30...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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