Key features and details
- Rabbit polyclonal to NuMA
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NuMA antibody
See all NuMA primary antibodies
DescriptionRabbit polyclonal to NuMA
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human NuMA aa 2065-2115. The exact sequence is proprietary. (NP_006176.2).
Database link: Q14980
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241470 was affinity purified using an epitope specific to NuMA immobilized on solid support.
Our Abpromise guarantee covers the use of ab241470 in the following tested applications.
|WB||1/2000 - 1/10000. Predicted molecular weight: 238 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be a structural component of the nucleus.
Cellular localizationNucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
- Information by UniProt
- Centrophilin stabilizes mitotic spindle in mitotic cells antibody
- NMP 22 antibody
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Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for NuMA using ab241470 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
All lanes : Anti-NuMA antibody (ab241470) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 238 kDa
Exposure time: 30 seconds
NuMA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241470 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241470 at 0.1 µg/ml.
Lane 1: ab241470 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab241470 has not yet been referenced specifically in any publications.