Overview

  • Product name

  • Description

    Rabbit polyclonal to NuMA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Common marmoset
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 900 and 950 of Human NuMA (NP_006176.2).

  • Positive control

    • Human Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Lung Adenocarcinoma, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Skin Melanoma, Stomach Adenocarcinoma and Testicular Seminoma.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

Target

  • Function

    May be a structural component of the nucleus.
  • Cellular localization

    Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • Database links

  • Alternative names

    • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
    • NMP 22 antibody
    • Nuclear matrix protein 22 antibody
    • Nuclear mitotic apparatus protein 1 antibody
    • Nuclear mitotic apparatus protein antibody
    • NUMA 1 antibody
    • NUMA antibody
    • NuMA protein antibody
    • NUMA1 antibody
    • NUMA1_HUMAN antibody
    • SP H antigen antibody
    • SP-H antigen antibody
    • Structural nuclear protein antibody
    see all

Images

  • ab84680 staining NuMA in Marmoset (common) testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Buoin's fixative, permeabilized in Triton X-100 (1/100 in PBS) and blocked with 25% serum for 30 minutes; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/100 in PBS + 0.5% BSA) for 17 hours at 4°C. A Biotin-conjugated Chicken anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • ab84680, at 1/100 dilution, staining NuMA in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.
  • ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using DAB staining.
  • ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry using DAB staining.
  • ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human ovarian tumor by Immunohistochemistry using DAB staining.

References

This product has been referenced in:

See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 25%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer (pH 6,0)
Sample
Marmoset (common) Tissue sections (Testis)
Specification
Testis
Permeabilization
Yes - Triton X-100 (1:100 in PBS) for 30 min
Fixative
Buoin's fixative

Abcam user community

Verified customer

Submitted Nov 26 2013

Answer

Thank you for contacting us.

At the site recognized by the antibody the human and mouse sequences are too divergent to expect binding. This antibody is not a good candidate for use in mouse although it has not been tested.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thanks for your enquiry.

Regarding the immunogen information:
ab5675 immunogen is Live Ls 174T cells and epitope is not known.

RE: could you provide Ab86129 and/or Ab84680 without BSA?
These 2 antibodies are not typically available in a formulation without BSA. There are 2 alternatives to remove the BSA.

1. For bulk orders we can typically provide you with a custom formulation. A bulk order would require purchase of at least 10 vials of antibody. Bulk orders are provided at a discount. For further information regarding bulk orders please contact Andrea Gray at mailto:Andrea.Gray@abcam.com.

2. We have a protein purification kit that would allow you to remove the BSA containing buffer provided with the antibody and replace it with a buffer of your choosing. Please see our product https://www.abcam.com/Antibody-Purification-Kit-Protein-A-ab102784.htmlfor more details.

I hope this is helpful. Please contact me again if you have any further questions.

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