• Product name

  • Description

    Rabbit polyclonal to NuMA
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human NuMA aa 900-1000 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab109067)

  • Positive control

    • This antibody gave a positive signal in MCF7 whole cell lysate.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab86129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 235 kDa (predicted molecular weight: 238 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Function

    May be a structural component of the nucleus.
  • Cellular localization

    Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • Database links

  • Alternative names

    • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
    • NMP 22 antibody
    • Nuclear matrix protein 22 antibody
    • Nuclear mitotic apparatus protein 1 antibody
    • Nuclear mitotic apparatus protein antibody
    • NUMA 1 antibody
    • NUMA antibody
    • NuMA protein antibody
    • NUMA1 antibody
    • NUMA1_HUMAN antibody
    • SP H antigen antibody
    • SP-H antigen antibody
    • Structural nuclear protein antibody
    see all


  • ICC/IF image of ab86129 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86129, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 anf MCF7 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.
  • Anti-NuMA antibody (ab86129) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 238 kDa
    Observed band size: 235 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 171 kDa, 35 kDa, 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 16 minutes

    Abcam recommends using a transfer buffer including 20% Ethanol and SDS as well as unheating tissue lysates for this product. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • IHC image of NuMA staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86129, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times


ab86129 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (U2-OS)
Yes - 0.1% Triton X-100 3x5min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 11 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Rat Tissue sections (Human Cells Transplanted into Rat Tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6, citrate based
Human Cells Transplanted into Rat Tissue
Blocking step
Serum-Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C

Premier Laboratory

Verified customer

Submitted Mar 25 2016


Thanks for your enquiry.

Regarding the immunogen information:
ab5675 immunogen is Live Ls 174T cells and epitope is not known.

RE: could you provide Ab86129 and/or Ab84680 without BSA?
These 2 antibodies are not typically available in a formulation without BSA. There are 2 alternatives to remove the BSA.

1. For bulk orders we can typically provide you with a custom formulation. A bulk order would require purchase of at least 10 vials of antibody. Bulk orders are provided at a discount. For further information regarding bulk orders please contact Andrea Gray at mailto:Andrea.Gray@abcam.com.

2. We have a protein purification kit that would allow you to remove the BSA containing buffer provided with the antibody and replace it with a buffer of your choosing. Please see our product https://www.abcam.com/Antibody-Purification-Kit-Protein-A-ab102784.htmlfor more details.

I hope this is helpful. Please contact me again if you have any further questions.

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