Overview

  • Product name

  • Description

    Rabbit polyclonal to NuMA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 309 of Human NuMA (AAH04165).

  • Positive control

    • 293T, A431, H1299, HeLa, MOLT4 or Raji cell lines.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 238 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function

    May be a structural component of the nucleus.
  • Cellular localization

    Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • Database links

  • Alternative names

    • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
    • NMP 22 antibody
    • Nuclear matrix protein 22 antibody
    • Nuclear mitotic apparatus protein 1 antibody
    • Nuclear mitotic apparatus protein antibody
    • NUMA 1 antibody
    • NUMA antibody
    • NuMA protein antibody
    • NUMA1 antibody
    • NUMA1_HUMAN antibody
    • SP H antigen antibody
    • SP-H antigen antibody
    • Structural nuclear protein antibody
    see all

Images

  • ab97585, at 1/200, staining paraformaldehyde-fixed A431 cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).
  • Anti-NuMA antibody (ab97585) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 238 kDa



    7.5% SDS PAGE
  • ab97585, at 1/100, staining paraffin-embedded Cal27 Xenograft by Immunohistochemistry.

References

This product has been referenced in:

  • Millar MW  et al. The Adhesion G-Protein-Coupled Receptor, GPR56/ADGRG1, Inhibits Cell-Extracellular Matrix Signaling to Prevent Metastatic Melanoma Growth. Front Oncol 8:8 (2018). Read more (PubMed: 29450192) »
  • Burgess HM  et al. Targeting Poxvirus Decapping Enzymes and mRNA Decay to Generate an Effective Oncolytic Virus. Mol Ther Oncolytics 8:71-81 (2018). Read more (PubMed: 29888320) »
See all 9 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Melanoma 5B1 cell line)
Permeabilization
Yes - Triton X100 0.1%
Specification
Melanoma 5B1 cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 14 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample
Human Tissue sections (Engineerd construct)
Specification
Engineerd construct
Permeabilization
No
Fixative
Paraformaldehyde

Mr. Hendrik Gremmels

Verified customer

Submitted Jan 16 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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