Anti-NuMA antibody [SPM300] - BSA and Azide free (ab212689)

Overview

  • Product name

    Anti-NuMA antibody [SPM300] - BSA and Azide free
    See all NuMA primary antibodies
  • Description

    Mouse monoclonal [SPM300] to NuMA - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human NuMA. (Colon carcinoma 174T cells).

  • Positive control

    • IHC-P: Human tonsil tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purification notes

    ab212689 was purified form bioreactor concentrate by PEG precipitation.
  • Clonality

    Monoclonal
  • Clone number

    SPM300
  • Isotype

    IgM
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab212689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

Target

  • Function

    May be a structural component of the nucleus.
  • Cellular localization

    Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • Database links

  • Alternative names

    • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
    • NMP 22 antibody
    • Nuclear matrix protein 22 antibody
    • Nuclear mitotic apparatus protein 1 antibody
    • Nuclear mitotic apparatus protein antibody
    • NUMA 1 antibody
    • NUMA antibody
    • NuMA protein antibody
    • NUMA1 antibody
    • NUMA1_HUMAN antibody
    • SP H antigen antibody
    • SP-H antigen antibody
    • Structural nuclear protein antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for NuMA using ab212689 at 1 µg/ml in immunohistochemical analysis.

    This IHC-P data was generated using the same anti-NuMA antibody clone, SPM300, in a different buffer formulation.

References

ab212689 has not yet been referenced specifically in any publications.

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