Anti-NUMB antibody (ab155415)
Key features and details
- Rabbit polyclonal to NUMB
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NUMB antibody
See all NUMB primary antibodies -
Description
Rabbit polyclonal to NUMB -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-244 of Human NUMB (Uniprot: P49757)
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General notes
Keep as a concentrated solutionReproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 71 kDa. |
Target
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Function
Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. -
Sequence similarities
Contains 1 PID domain. -
Post-translational
modificationsIsoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 512187 Cow
- Entrez Gene: 8650 Human
- Entrez Gene: 18222 Mouse
- Entrez Gene: 29419 Rat
- Entrez Gene: 100038147 Xenopus tropicalis
- Omim: 603728 Human
- SwissProt: P49757 Human
- SwissProt: Q9QZS3 Mouse
see all -
Alternative names
- c14_5527 antibody
- C14orf41 antibody
- FLJ31314 antibody
see all
Images
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Western blot - Anti-NUMB antibody (ab155415)Anti-NUMB antibody (ab155415) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 71 kDa
7.5% SDS PAGE
References (1)
ab155415 has been referenced in 1 publication.
- Wang R & Liu C All-trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line. Oncol Lett 18:3646-3654 (2019). PubMed: 31579077
