Product nameAnti-NUMB antibody
See all NUMB primary antibodies
DescriptionRabbit polyclonal to NUMB
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Synthetic peptide within Human NUMB aa 375-425. The exact sequence is proprietary.
Database link: P49757
- WB: HeLa and HEK-293T whole cell lysate, IP: HeLa cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000.|
FunctionPlays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
Sequence similaritiesContains 1 PID domain.
modificationsIsoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
- Information by UniProt
- c14_5527 antibody
- C14orf41 antibody
- FLJ31314 antibody
NUMB was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264224 at 3 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab264224 at 0.1 µg/ml.
Lane 1: ab264224 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
All lanes : Anti-NUMB antibody (ab264224) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264224 has not yet been referenced specifically in any publications.