Key features and details
- Rabbit polyclonal to NUMB
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NUMB antibody
See all NUMB primary antibodies
DescriptionRabbit polyclonal to NUMB
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human NUMB aa 525-575. The exact sequence is proprietary.
Database link: P49757
- IP: HeLa whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Use at 2-5 µg/mg of lysate.
FunctionPlays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
Sequence similaritiesContains 1 PID domain.
modificationsIsoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
- Information by UniProt
- c14_5527 antibody
- C14orf41 antibody
- FLJ31314 antibody
NUMB was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264225 at 3 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab264224 at 0.1 µg/ml.
Lane 1: ab264225 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264225 has not yet been referenced specifically in any publications.