For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat Tissue sections (Brain sections)
Antigen retrieval step
Other product details
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3 % Triton X100
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 488
Host species: Goat
Conjugation: Alexa Fluor® 488
The antibody produced a nice staining in many fibers or synapses in many brain areas.
The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
Dr. Sophie Pezet
Submitted Jun 05 2009